Chronic oral administraton of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart

Title:

Chronic oral administraton of beta-adrenoceptor agonist clenbuterol affects myosin heavy chain (MHC) expression in adult mouse heart

Creator:

Patiyal, Som N. and Sharma, S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:beac2a79-f9bf-4982-aea7-6572ce3b2d1b
uuid:beac2a79-f9bf-4982-aea7-6572ce3b2d1b
issn:0862-8408

Subject:

Biologické vědy, fyziologie, srdce, physiology, heart, clenbuterol, myosin heavy chain isoforms, left ventricle, actomyosin ATPase, cardiac muscle contraction, 2, and 57/59

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The aim of this study was to analyze the effects of chronic administration of the β-adrenoceptor agonist clenbuterol (2 mg/kg body weight/day for a period of 30 days) on the major contractile protein (myosin) in the left ventricular muscle of the adult mouse heart. Separation of myosin heavy chain (MHC) isoforms on 7.5 % glycerol SDS-PAGE and subsequent quantification of the gels by laser densitometry showed a 6.5-fold increase in the β-isoform of MHC in the clenbuterol-treated group. The α: β ratio of these two isoforms in the control group was 98.16±0.14 %: 1.83±0.14 %, whereas in the treated group it was 88.05±1.15 %: 11.95±1.15 %. Actomyosin ATPase activity assay demonstrated a significant (20 %) decline in ATPase activity of the tissue in the β-agonist-treated group. These results suggest that chronic clenbuterol treatment is capable to induced the transformation of MHC isoforms increasing the slow β-MHC isoform, which may contribute to the altered contractile mechanics of clenbuterol-treated hearts., S. N. Patiyal, S. Sharma., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:3

Harvested from:

CDK

Metadata only:

false