Myotropic neuropeptides from the retrocerebral complex of the stick insect, Carausius morosus (Phasmatodea: Lonchodidae)

Title:

Myotropic neuropeptides from the retrocerebral complex of the stick insect, Carausius morosus (Phasmatodea: Lonchodidae)

Creator:

Predel, Reinhard, Kellner, Roland, and Gäde, Gerd

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bf929cf2-abf8-4c00-8aac-1632bd97ebee
uuid:bf929cf2-abf8-4c00-8aac-1632bd97ebee

Subject:

zoologie, hmyz, Phasmatodea, Lonchodidae, Carausius morosus, neuropeptidy, myotropin, myotropické neuropeptidy, izolace, bioaktivita, 595.2/.7, and 591.1

Type:

article, bibliography, model:article, and TEXT

Format:

print, text, and regular print

Description:

Myotropic neuropeptides were isolated from the retrocerebral complex of the stick insect, Carausius morosus, by using three HPLC steps. Bioactivity during purification was measured by heterologous bioassays monitoring the contractions of the hyperneural muscle and hindgut of the American cockroach. Additionally, fractions not active in these bioassays were tested in a homologous bioassay evoking contractions of the hindgut of C. morosus. Peptide sequence analysis and mass spectrometry yielded the following structures: Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2 (CCAP), pGlu-Thr-Phe-Gln-Tyr-Ser-His-Gly-Trp-Thr-Asn-NH2 (His7-corazonin) and Asp-Glu-Gly-Gly-Thr-Gln-Tyr-Thr-Pro-Arg-Leu-NH2 (Cam-PK-1). These neuropeptides are the first myotropins isolated from C. morosus. The most bioactive compound in the homologous bioassay, the C. morosus-hindgut assay, was CCAP., Reinhard Predel, Roland Kellner, Gerd Gäde, and Lit

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 1999 Volume:96 | Number:3

Harvested from:

CDK

Metadata only:

false