Detection of toxoplasmosis in environmental samples at a wet market of a capital city centre

Title:

Detection of toxoplasmosis in environmental samples at a wet market of a capital city centre
Detection of toxoplasmosis in environmental samples at a wet market of a capital city centre

Creator:

Nimir, Amal R. and Linn,Tang Cher

Contributor:

Nimir, Amal R. and Linn,Tang Cher

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc12000517-c13c3bb5-dcf1-4f12-9022-f29ad6e40a0a
uuid:bmc12000517-c13c3bb5-dcf1-4f12-9022-f29ad6e40a0a
local:bmc12000517
http://www.lfhk.cuni.cz/Data/files/Casopisy/2011/2011_107.pdf
local: bmc12000517

Subject:

Toxoplasma--izolace a purifikace, toxoplazmóza--epidemiologie, toxoplazmóza zvířat--diagnóza, mikrobiologie vody, krysy--parazitologie, mozek--parazitologie, and parazitární zátěž--klasifikace--využití

Type:

model:article, article, Text, and TEXT

Format:

braille, text, and regular print

Description:

The local Chow Kit market is the largest wet market in the city of Kuala Lumpur. It is very close to the biggest government hospital in the city centre. However, the level of cleanliness in this area is always questionable and a matter of concern. The aim of this study was to identify the prevalence of T. gondii oocyst in water samples used by hawkers in that market and tissue cysts in rats’ brains captured from the same area. Water samples were taken to the parasitology laboratory at the National Universtiy of MalaysiaUniversity and a sugar flotation concentration method was used. Supernatant microscopical examination was then performed. A total of 752 slides were screened for the presence of T. gondii oocyst. A hundred rats wandering in the same area were also captured by the hawkers using mousetraps. After each animal was sacrificed, and an electric microtome was used to cut out serial sections 5μ thick from the rat brains. The de-waxed tissue sections were stained by the progressive Haematoxylin and Eosin (H&E) stain for microscopical examination. A total of 1000 slides were screened under a light microscope to detect the presence of T. gondii brain cysts. All the water samples were found to be negative for T. gondii oocyst. Out of the 100 rats captured, three rats were found to possess T. gondii cysts in their brains. Water samples reflect minimal or no solid food contamination, while the 3% of positive brain cysts influence the researchers to broaden their investigations for future projects., Amal R. Nimir, Tang Cher Linn, and Literatura 16

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové)--MED00010947

Source:

Acta medica (Hradec Králové) | 2011 Volume:54 | Number:3

Harvested from:

CDK

Metadata only:

false

Date:

2011