Quantitative analysis of cerebrospinal fluid brain derived neurotrophic factor in the patients with multiple sclerosis

Title:

Quantitative analysis of cerebrospinal fluid brain derived neurotrophic factor in the patients with multiple sclerosis
Quantitative analysis of cerebrospinal fluid brain derived neurotrophic factor in the patients with multiple sclerosis

Creator:

Mashayekhi, Farhad, Salehi, Zivar, and Jamalzadeh, Hamid Reza

Contributor:

Mashayekhi, Farhad, Salehi, Zivar, and Jamalzadeh, Hamid Reza

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc12036487-1079b7b2-2154-41ab-8f9b-fa4c2940a6d6
uuid:bmc12036487-1079b7b2-2154-41ab-8f9b-fa4c2940a6d6
local:bmc12036487
http://www.lfhk.cuni.cz/article.asp?nArticleID=6944&nLanguageID=2
local: bmc12036487

Subject:

dospělí, mozkový neurotrofický faktor--mok mozkomíšní, ženské pohlaví, lidé, mužské pohlaví, and roztroušená skleróza--mok mozkomíšní

Type:

model:article, article, Text, and TEXT

Format:

braille, text, and regular print

Description:

Multiple sclerosis (MS) is the most common cause of nontraumatic neurological disability in Europe and North America. Growth factor expression could participate in the repair process of the demyelinating disease. Among growth factors, brain derived neurotrophic factors (BDNF) has been demonstrated to play an important role in neuronal and axonal survival. In the central nervous system (CNS ), neurons are the main source of BDNF. Another potential source are activated astrocytes, which are present in inflamed areas in the CNS as shown in MS. In this study, total protein concentration (TPC) and BDNF levels in the cerebrospinal fluid (CS F) samples from the patients with MS (n = 48) and control subjects (n = 53) were measured using a Bio-Rad protein assay and enzyme linked immunosorbent assay (ELISA). No significant change in the CS F TPC of patients with MS was seen as compared to normal CS F. The presence of BDNF in the CS F samples was shown by Western blot. Using ELISA , it was shown that the level of BDNF in the MS CS F is higher than in normal CS F. It is concluded that BDNF is a constant component of human CS F. Moreover, it could be implicated in the pathophysiology of MS., Farhad Mashayekhi, Zivar Salehi, Hamid Reza Jamalzadeh, and Literatura 27

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové)--MED00010947

Source:

Acta medica (Hradec Králové) | 2012 Volume:55 | Number:2

Harvested from:

CDK

Metadata only:

false

Date:

2012