Antiproliferative effects of selected chemotherapeutics in human ovarian cancer cell line A2780

Title:

Antiproliferative effects of selected chemotherapeutics in human ovarian cancer cell line A2780
Antiproliferative effects of selected chemotherapeutics in human ovarian cancer cell line A2780

Creator:

Caltová, Kateřina and Červinka, Miroslav

Contributor:

Caltová, Kateřina and Červinka, Miroslav , 1950-

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc12040502-c22e2ced-5155-499f-a169-9a825d1cdbf6
uuid:bmc12040502-c22e2ced-5155-499f-a169-9a825d1cdbf6
local:bmc12040502
http://www.lfhk.cuni.cz/article.asp?nArticleID=4434&nDepartmentID=1288&nLanguageID=2
local: bmc12040502

Subject:

nádory vaječníků--farmakoterapie, lidé, ženské pohlaví, buněčné linie nádorové, incidence, cytostatické látky--aplikace a dávkování--terapeutické užití, kombinovaná farmakoterapie, antitumorózní látky--klasifikace, cisplatina, paclitaxel, karboplatina, deoxycytidin, protokoly antitumorózní kombinované chemoterapie, topotekan, etoposid, statistika jako téma, buněčný cyklus, buňky - fyziologické jevy, proliferace buněk--účinky léků, and čas

Type:

model:article, article, Text, práce podpořená grantem, and TEXT

Format:

braille, text, and regular print

Description:

The aim of our study was to determine the effect of selected cytostatics on a human ovarian cancer cell line A2780 as a model system for ovarian cancer treatment. This cell line is considered cisplatin-sensitive. Panel of tested cytostatics included cisplatin, paclitaxel, carboplatin, gemcitabine, topotecan and etoposide. These cytostatics have a different mechanism of action. To evaluate cytotoxic potential of the tested compounds, the methods measuring various toxicological endpoints were employed including morphological studies, MTT assay, dynamic monitoring of cell proliferation with xCELLigence, cell cycle analysis, caspase 3 activity and expression of proteins involved in cell cycle regulation and cell death. The A270 cell line showed different sensitivity towards the selected cytostatics, the highest cytotoxic effect was associated with paclitaxel and topotecan., Kateřina Caltová, Miroslav Červinka, and Literatura 38

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové)--MED00010947

Source:

Acta medica (Hradec Králové) | 2012 Volume:55 | Number:3

Harvested from:

CDK

Metadata only:

false

Date:

2012