Human plasma and human platelet-rich plasma as a substitute for fetal calf serum during long-term cultivation of mesenchymal dental pulp stem cells

Title:

Human plasma and human platelet-rich plasma as a substitute for fetal calf serum during long-term cultivation of mesenchymal dental pulp stem cells
Human plasma and human platelet-rich plasma as a substitute for fetal calf serum during long-term cultivation of mesenchymal dental pulp stem cells

Creator:

Suchánková Kleplová, Tereza, Soukup, Tomáš, Řeháček, Vít, and Suchánek, Jakub

Contributor:

Suchánková Kleplová, Tereza, Soukup, Tomáš , 1980-, Řeháček, Vít, and Suchánek, Jakub , 1981-

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc15020402-a6044693-93ff-4f35-8b26-6b0d9a1d4ffe
uuid:bmc15020402-a6044693-93ff-4f35-8b26-6b0d9a1d4ffe
local:bmc15020402
http://actamedica.lfhk.cuni.cz/
doi: 10.14712/18059694.2014.50
local: bmc15020402

Subject:

zvířata, skot, buněčné kultury--metody, proliferace buněk, buňky kultivované, kultivační média, zubní dřeň--cytologie, fetální krev, lidé, mezenchymální stromální buňky, plazma bohatá na destičky, and čas

Type:

model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT

Format:

print, text, and regular print

Description:

AIMS: Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood components, and subsequently to investigate their basic biological properties. METHODS: DPSC were cultivated in five different media based on α MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically. RESULTS: We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 ± 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 ± 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 ± 10.4 hours), 2% PRP (DT 40.1 ± 5.7 hours) and 2% HP (DT 49.0 ± 15.2 hours) showed almost the same proliferative activity. DPSC's viability in the 9th passage was over 90% except for the DPSC cultivated in the 10% HP media. CONCLUSIONS: We proved that human blood components are suitable substitution for FCS in cultivation media for long-term DPSC cultivation. and T. Suchánková Kleplová, T. Soukup, V. Řeháček, J. Suchánek

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové) Universitas Carolina, Facultas Medica Hradec Králové--MED00010947

Source:

Acta medica (Hradec Králové) | 2014 Volume:57 | Number:3

Harvested from:

CDK

Metadata only:

false

Date:

2014