Natural detoxification capacity to inactivate nerve agents Sarin and VX in the rat blood

Title:

Natural detoxification capacity to inactivate nerve agents Sarin and VX in the rat blood
Natural detoxification capacity to inactivate nerve agents Sarin and VX in the rat blood

Creator:

Bajgar, Jiří, Cabal, Jiří, Kassa, Jiří, and Pavlík, Michal

Contributor:

Bajgar, Jiří , 1944-, Cabal, Jiří , 1957-, Kassa, Jiří , 1956-, and Pavlík, Michal

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc16019679-48622c1d-9295-48fa-a506-106e33e28ac1
uuid:bmc16019679-48622c1d-9295-48fa-a506-106e33e28ac1
local:bmc16019679
http://actamedica.lfhk.cuni.cz/
doi: 10.14712/18059694.2016.4
local: bmc16019679

Subject:

zvířata, inhibitory cholinesteráz--farmakokinetika--toxicita, cholinesterasy--metabolismus, ženské pohlaví, metabolická inaktivace, otrava organofosfáty--metabolismus, organothiofosforové sloučeniny--farmakokinetika--toxicita, krysy, potkani Wistar, and sarin--farmakokinetika--toxicita

Type:

model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT

Format:

print, text, and regular print

Description:

BACKGROUND: The method of continual determination of the rat blood cholinesterase activity was developed to study the changes of the blood cholinesterases following different intervetions. AIMS: The aim of this study is registration of cholinesterase activity in the rat blood and its changes to demonstrate detoxification capacity of rats to inactivate sarin or VX in vivo. METHODS: The groups of female rats were premedicated (ketamine and xylazine) and cannulated to a. femoralis. Continual blood sampling (0.02 ml/min) and monitoring of the circulating blood cholinesterase activity were performed. Normal activity was monitored 1-2 min and then the nerve agent was administered i.m. (2×LD50). Using different time intervals of the leg compression and relaxation following the agent injection, cholinesterase activity was monitored and according to the inhibition obtained, detoxification capacity was assessed. RESULTS: Administration of sarin to the leg, then 1 and 5 min compression and 20 min later relaxation showed that further inhibition in the blood was not observed. On the other hand, VX was able to inhibit blood cholinesterases after this intervention. CONCLUSIONS: The results demonstrated that sarin can be naturally detoxified on the contrary to VX. Described method can be used as model for other studies dealing with changes of cholinesterases in the blood following different factors. and J. Bajgar, J. Cabal, J. Kassa, M. Pavlík

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové) Universitas Carolina, Facultas Medica Hradec Králové--MED00010947

Source:

Acta medica (Hradec Králové) | 2015 Volume:58 | Number:4

Harvested from:

CDK

Metadata only:

false

Date:

2015