Pyridine nucleotide contents and activity of protochlorophyliide photooxidoreductase in vivo. 2. Comparative investigations in protoplasts and etioplasts of Avena sativa L. during greening

Title:

Pyridine nucleotide contents and activity of protochlorophyliide photooxidoreductase in vivo. 2. Comparative investigations in protoplasts and etioplasts of Avena sativa L. during greening

Creator:

Peine, G., Pilz, K., Walter, G., Dujardin, E. C., and Hoffmann, P.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c44cb1b8-4a9e-4790-a030-a58223d094b5
uuid:c44cb1b8-4a9e-4790-a030-a58223d094b5
urnnbn:urn:nbn:cz:aba007-000ffw

Type:

model:internalpart and TEXT

Format:

print

Description:

Etiolated oat mesophyll protoplasts and etioplasts released from etiolated protoplasts were able to perform protochlorophyliide (PChlide) reduction but showed a high amount of inactive P633/628» compared with intact leaves. In špite of this, the anabolic reduction charge (ARC, NAPDH+H+/NADP+ + NADPF1+H+) was maintained at 0.66 - 0.77 up to 30 min of irradiation with weak "white light" (10 W m‘2). This indicated a high buffering capacity of the isolated systém to provide NADPF1+H+, only at the expense of intemal reserves. A change in the redox statě of the NADP+/NADPH+H^ couple during protochlorophyliide reduction was not observed in etioplasts. The resulting redox potential was in the range of -330 mV optimal for protochlorophyliide reduction.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

13-24 and 15-26

Source:

Photosynthetica | 1993 Volume:29 | Number:1

Harvested from:

CDK

Metadata only:

false