In vitro changes in secretion activity of rat ovarian fragments induced by molybdenum

Title:

In vitro changes in secretion activity of rat ovarian fragments induced by molybdenum

Creator:

Roychoudhury, S., Detvanova, L., Alexander V. Sirotkin, Toman, R., and Kolesarova, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c4c77f39-378f-4c14-b887-6e89306d25a0
uuid:c4c77f39-378f-4c14-b887-6e89306d25a0
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, molybden, progesteron, molybdenum, progesterone, rat ovary, estradiol, IGF-I, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The aim of this in vitro study was to examine the secretion activity (progesterone, 17β-estradiol and insulin-like growth factor-I) of rat ovarian fragments after molybdenum (Mo) addition. Rat ovarian fragments were incubated with ammonium molybdate (NH4)6Mo7O24.4H2O at the doses 90, 170, 330 and 500 μg.ml-1 for 24 h and compared with control group without Mo addition. Release of progesterone (P4), estradiol (17β-estradiol) and insulin-like growth factor I (IGF-I) by ovarian fragments was assessed by radioimmunoassay (RIA). Data show that P4 release by ovarian fragments was not affected by (NH4)6.Mo7O24.4H2O addition at all the doses used (90-500 μg.ml-1). However, addition of ammonium molybdate was found to cause a significant (P<0.05) dose-dependent decrease (at the doses 90, 170 and 500 μg.ml-1) in release of 17β-estradiol by ovarian fragments in comparison to control. Also, addition of ammonium molybdate significantly (P<0.05) inhibited IGF-I release at all the doses (90-500 μg.ml-1) used in the study. Results suggest ammonium molybdate induced inhibition in the release of growth factor IGF-I and its dosedependent effect on secretion of steroid hormone 17β-estradiol but not progesterone. These data contribute to new insights regarding the mechanism of action of Mo on rat ovarian functions., S. Roychoudhury, L. Detvanova, A. V. Sirotkin, R. Toman, A. Kolesarova., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2014 Volume:63 | Number:6

Harvested from:

CDK

Metadata only:

false