Regulatory role of nitric oxide on the cardiac Na, K-ATPase in hypertension

Title:

Regulatory role of nitric oxide on the cardiac Na, K-ATPase in hypertension

Creator:

Vlkovičová, J., Veronika Javorková, Mézešová, L., Oľga Pecháňová, and Vrbjar, Norbert

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c659ee6c-810c-47ca-b915-9d82218f808f
uuid:c659ee6c-810c-47ca-b915-9d82218f808f
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, kardiovaskulární fyziologie, hypertenze, oxid dusnatý, srdce, cardiovascular physiology, hypertension, nitric oxide, heart, sodium pump, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The present study was focused on regulatory role of nitric oxide on functional properties of the cardiac Na, K-ATPase in three various animal models of hypertension: spontaneously hypertensive male rats (SHR) with increased activity of nitric oxide synthase (NOS) by 60 % (Sh1), SHR with decreased activity of NOS by 40 % (Sh2) and rats with hypertension induced by L-NAME (40 mg/kg/day) with depressed activity of NOS by 72 % (LN). Studying the utilization of energy substrate we observed higher Na, K-ATPase activity in the whole concentration range of ATP in Sh1 and decreased activity in Sh2 and LN. Evaluation of kinetic parameters revealed an increase of Vmax value by 37 % in Sh1 and decrease by 30 % in Sh2 and 17 % in LN. The KM value remained unchanged in Sh2 and LN, but was lower by 38 % in Sh1 indicating increased affinity of the ATP binding site, as compared to controls. During the activation with Na+ we observed increased Vmax by 64 % and increased KNa by 106 % in Sh1. In Sh2 we found decreased Vmax by 40 % and increased KNa by 38 %. In LN, the enzyme showed unchanged Vmax with increased KNa by 50 %. The above data indicate a positive role of increased activity of NOS in improved utilization of ATP as well as enhanced binding of Na+ by the cardiac Na, K-ATPase., J. Vlkovičová, V. Javorková, L. Mézešová, O. Pecháňová, N. Vrbjar., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:Suppl 2

Harvested from:

CDK

Metadata only:

false