Activation of adenylate cyclase system in the preconditioned rat heart

Title:

Activation of adenylate cyclase system in the preconditioned rat heart

Creator:

Ľudmila Okruhlicová, Ravingerová, T., Dezider Pancza, Narcisa Tribulová, Ján Styk, and Štetka, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c778cc67-607b-4745-9479-e1f5ef76332f
uuid:c778cc67-607b-4745-9479-e1f5ef76332f
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, cytochemie, srdce, cytochemistry, heart, ischemic preconditioning, adenylate cyclase, rat, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Ischemic preconditioning (IP) protects the heart against subsequent prolonged ischemia. Whether the β-adrenoceptor/adenylate cyclase pathway contributes to this cardioprotection is not yet fully known. Using enzyme catalytic cytochemistry we studied the adenylate cyclase activity and its distribution in the preconditioned rat heart. Adenylate cyclase activity was examined in Langendorff-perfused rat hearts subjected to the following conditions: control perfusion; 30 min regional ischemia; 5 min occlusion and 10 min reperfusion (IP); IP followed by ischemia. Ischemia-induced arrhythmias and the effect of ischemic preconditioning on the incidence of arrhythmias were analyzed. At the end of experiment the heart was shortly prefixed with glutaraldehyde. Tissue samples from the left ventricle were incubated in a medium containing the specific substate AMP-PNP for adenylate cyclase and then routinely processed for electron microscopy. Adenylate cyclase activity was cytochemically demonstrated in the sarcolemma and the junctional sarcoplasmic reliculum (JSR) in control hearts, while it was absent after test ischemia. The highest activity of the precipitate was observed after ischemic preconditioning. In the preconditioned hearts followed by test ischemia, adenylate cyclase activity in the precipitate was preserved in sarcolemma and even more in JSR. Protective effect of ischemic preconditioning was manifested by the suppression of severe arrhythmias. These rresults indicate the involvement of the adenylate cyclase system in mechanisms underlying ischemic preconditioning., Ľ. Okruhlicová, T. Ravingerová, D. Pancza, N. Tribulová, J. Styk, R. Štetka., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2000 Volume:49 | Number:2

Harvested from:

CDK

Metadata only:

false