Acute liver failure induced by thioacetamide : Selection of Optimal Dosage in Wistar and Lewis Rats: selection of optimal dosage in wistar and lewis rats

Title:

Acute liver failure induced by thioacetamide : Selection of Optimal Dosage in Wistar and Lewis Rats: selection of optimal dosage in wistar and lewis rats

Creator:

Eva Koblihová, Mrázová, I., Zdenka Vernerová, and Ryska, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c95dad6d-60c9-4d56-abaa-e7b723a0d554
uuid:c95dad6d-60c9-4d56-abaa-e7b723a0d554
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, játra, liver, acute liver failure, thioacetamide, wistar rats, lewis rats, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Acute liver failure (ALF) is a clinical condition with very high mortality rate. Its pathophysiological background is still poorly understood, which necessitates a search for optimal experimental ALF models with features resembling those of the human disorder. Taking into consideration reproducibility of induction of ALF, adequate animal size, cost of animals, the required time gap between insult and death of animals (“therapeutic window”), potential risk to investigator and other aspects, administration of thioacetamide (TAA) in rats is currently most recommended. However, the fundamental details of this ALF model have not yet been evaluated. This prompted us to investigate, first, the course of ALF as induced by intraperitoneal TAA at doses increasing from 175 to 700 mg/kg BW per day. The animals’ survival rate, plasma alanine and aspartate aminotransferase activities, and bilirubin and ammonia levels were determined over the follow-up period. Second, we examined whether Wistar and Lewis rats exhibit any differences in the course of ALF induced by different TAA doses. We found that the optimal dose for ALF induction in rats is 350 mg.kg-1 i.p., given as a single injection. Wistar rats proved more susceptible to the development of TAA-induced ALF compared with Lewis rats. Collectively, our present findings provide a sound methodological background for experimental studies aimed at evaluation of pathophysiology and development of new approaches in the therapy of ALF., E. Koblihová, I. Mrázová, Z. Vernerová, M. Ryska., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2014 Volume:63 | Number:4

Harvested from:

CDK

Metadata only:

false