Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia

Title:

Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia

Creator:

Naryzhnaya, N. V. , Khaliulin, I. , Lishmanov, Y. B. , Suleiman, M. S., Tsibulnikov, S. Y. , Kolar, F., and Maslov, L. N.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ccbeaaa2-0397-4211-bf29-3a11a6658d6d
uuid:ccbeaaa2-0397-4211-bf29-3a11a6658d6d
doi:10.33549/physiolres.933938

Subject:

Cardiomyocytes, Chronic hypoxia, Anoxia/reoxygenation, and Opioid receptors

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

We studied the role of the δ, µ, and к opioid receptor (OR) subtypes in the cardioprotective effect of chronic continuous normobaric hypoxia (CNH) in the model of acuteanoxia/ reoxygenation of isolated cardiomyocytes. Adaptation of rats to CNH was performed by their exposure to atmosphere containing 12 % of O2 for 21 days. Anoxia/reoxygenation of cardiomyocytes isolated from normoxic control rats caused the death of 51 % of cells and lactate dehydrogenase (LDH) release. Adaptation of rats to CNH resulted in the anoxia/reoxygenation-induced cardiomyocyte death of only 38 %, and reduced the LDH release. Pre-incubation of the cells with either the non-selective OR blocker naloxone (300 nM/l), the δ OR antagonist TIPP(ψ) (30 nM/l), the selective δ2 OR antagonist naltriben (1 nM/l) or the μ OR antagonist CTAP (100 nM/l) for 25 minutes before anoxia abolished the reduction of cell death and LDH release afforded by CNH. The antagonist of δ1 OR BNTX (1 nM/l) or the κ OR antagonist nor-binaltorphimine (3 nM/l) did not influence the cytoprotective effects of CNH. Taken together, the cytoprotective effect of CNH is associated with the activation of the δ2 and μ OR localized on cardiomyocytes.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

245-253

Source:

Physiological research | 2019 Volume:68 | Number:2

Harvested from:

CDK

Metadata only:

false