Collagenolytic potential of rat liver myofibroblasts

Title:

Collagenolytic potential of rat liver myofibroblasts

Creator:

Jiroutová, A., Peterová, E., Bittnerová, L., Rastislav Slavkovský, Čevelová, P., Martina Řezáčová, Jaroslav Cerman, Stanislav Mičuda, and Jiří Kanta

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:cd128a37-8b96-48f5-9291-adb4f394b202
uuid:cd128a37-8b96-48f5-9291-adb4f394b202

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, liver myofibroblasts, extracellular matrix, cytoskeleton, gene expression, matrix metalloproteinases, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Rat liver myofibroblasts (MFB) were isolated by repeated passaging of nonparenchymal liver cell fraction. They were cultured on polystyrene Petri dishes, on fibrin or on type I collagen gels for 5 days. Quantitative RT-PCR, Western blotting, zymography and immunocytochemistry were used to study differences in cell morphology and protein expression. MFB were large and spread on plastic substrate, with prominent α-smooth muscle (α-SMA) fibres. They turned much smaller and elongated on collagen which was accompanied by the rearrangement of the cytoskeleton and a decrease in α-SMA and β-actin content. Collagen gel induced the expression of a group of metalloproteinases (MMP-2, -3, -9, -13), on mRNA and protein level which resulted in the degradation of the gel. This response was accompanied by changes in the mRNA expression of cytokines of TGF-β family, CTGF and interleukin-6, as well as of osteopontin and thrombospondin-2 that are involved in metalloproteinases (MMPs) regulation. The expression of MMPs substrates, collagen types I, IV and XII did not change or decreased. The effects of fibrin gels on MFB were milder than those of collagen. MFB assumed to deposit collagen and other ECM components in fibrotic liver, besides hepatic stellate cells, also possess a great collagenolytic potential., A. Jiroutová, ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2013 Volume:62 | Number:1

Harvested from:

CDK

Metadata only:

false