The H19/let-7 feedback loopcontributes to developmentaldysplasia and dislocationofthe hip.

Title:

The H19/let-7 feedback loopcontributes to developmentaldysplasia and dislocationofthe hip.

Creator:

Ning, Bo, Jin, Rui, Wang, Dahui , and Sun, Jun

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:cf0016d6-6582-4074-9829-b686a5a718bf
uuid:cf0016d6-6582-4074-9829-b686a5a718bf
doi:10.33549/physiolres.933920

Subject:

Developmental dysplasia and dislocation of hip, H19, Proliferation, and let-7

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Developmental dysplasia and dislocation of the hip (DDH) is the most common type of lower limb deformity in pediatric orthopedics. The mechanism of the signaling pathway has been studied in depth. However, the role of epigenetic regulation, such as lncRNA, is still far from clear. In this study, we successfully established a rat model of DDH and demonstrated that H19 was down-regulated in the development of DDH. Further, we constructed H19 knockdown (KD) and overexpression chondrocytes. H19 KD suppressed the proliferation of normal chondrocytes, while overexpression of H19 promoted cell proliferation of DDH chondrocytes. Finally, we revealed that H19 bound to let-7 and inhibited its function, acting as a competing endogenous RNA. Down-regulation of H19 is closely associated with DDH progression and H19 is an important epigenetic factor that regulates the proliferation of chondrocytes. H19 may thus be a potential clinical marker for DDH diagnosis and treatment.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

275-284

Source:

Physiological research | 2019 Volume:68 | Number:2

Harvested from:

CDK

Metadata only:

false