Multinucleate host cells induced by Vittaforma corneae (Microsporidia)

Title:

Multinucleate host cells induced by Vittaforma corneae (Microsporidia)

Creator:

Leitch, Gordon J., Shaw, Andrew P., Colden-Stanfield, Margaret, Scanlon, Mary, and Visvesvara, Govinda S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d23dbf1a-789d-46c9-b931-5fe69f7e6e6d
uuid:d23dbf1a-789d-46c9-b931-5fe69f7e6e6d
doi:10.14411/fp.2005.013

Subject:

Vittaforma corneae, microsporidia, microtubule organizing centre, host-parasite interaction, and multinucleate giant cell

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The microsporidium Vittaforma corneae (Shadduck, Meccoli, Davis et Font, 1990) develops within the target cell cytoplasm. In the present study, green monkey kidney (E6) cells infected at 30°C, 35°C or 37°C with V. corneae developed enlarged multinucleate structures of up to 200 µm in any horizontal dimension made up either of a single cell or of multiple fused cells. A number of epithelial cell types (SW-480, HT-29, Caco-2 and HCT-8) were infected with V. corneae but did not induce the same highly organized structures, suggesting that for the structure to develop, the host cell must be capable of continued mitosis, and not be differentiated or be detaching from the surface matrix. Live cell imaging of infected E6 cells revealed large, multinucleate infected cells characterized by a central focus from which radiated parasite stages and host cell mitochondria. Immunocytochemistry identifying γ and α tubulin suggested that a single centrally-located microtubule organizing centre governed the distribution of parasite stages and host cell organelles, with mitochondria and parasites being eventually transported towards the periphery of the structure. Whole cell patch clamp analysis of infected cells indicated an average five-fold increase in total membrane capacitance, consistent with an enlarged single cell. Scanning electron microscopy revealed cell-like protrusions around the periphery of the structure with the intervening space being made up of parasites and cell debris. Clearly in the case of V. corneae-infected E6 cells the parasite-host cell relationship involves subverting the host cell cytoskeleton and cell volume control, providing the parasite with the same protected niche as does a xenoma.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

103-110

Source:

Folia parasitologica | 2005 Volume:52 | Number:1-2

Harvested from:

CDK

Metadata only:

false