L-malate enhances the gene expression of carried proteins and antioxidant enzymes in liver of aged rats

Title:

L-malate enhances the gene expression of carried proteins and antioxidant enzymes in liver of aged rats

Creator:

Zeng, X., Wu, J., Wu, Q., and Zhang, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d474acff-2686-4b67-a245-7cf370846453
uuid:d474acff-2686-4b67-a245-7cf370846453
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, l-malate, mitochondria antioxidant, malate-aspartate shuttle, real-time reverse transcription polymerase chain reaction (real-time RT-PCR), 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Previous studies in our laboratory reported L-malate as a free radical scavenger in aged rats. To investigate the antioxidant mechanism of L-malate in the mitochondria, we analyzed the change in gene expression of two malate-aspartate shuttle (MAS)-related carried proteins (A GC, aspartate/glutamate carrier and OMC, oxoglutarate/malate carrier) in the inner mitochondrial membrane, and three antioxidant enzymes (CAT, SOD, and GSH-Px) in the mitochondria. The changes in gene expression of these proteins and enzymes were examined by real-time RT-PCR in the heart and liver of aged rats treated with L-malate. L-malate was orally administered in rats continuously for 30 days using a feeding atraumatic needle. We found that the gene expression of OMC and GSH-Px mRNA in the liver increased by 39 % and 38 %, respectively, in the 0.630 g/kg L-malate treatment group than that in the control group. The expression levels of SOD mRNA in the liver increased by 39 %, 56 %, and 78 % in the 0.105, 0.210, and 0.630 g/kg L-malate treatment groups, respectively. No diffe rence were observed in the expression levels of AGC, OMC, CAT, SOD, and GSH-Px mRNAs in the heart of rats between the L-malate treatment and control groups. These results predicted that L-malate may increase the antioxidant capacity of mitochondr ia by enhancing the expression of mRNAs involved in the MAS and the antioxidant enzymes., X: Zeng, J. Wu, Q. Wu, J. Zhang., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:1

Harvested from:

CDK

Metadata only:

false