Protease-activated receptor-2 regulates glial scar formation via JNK signaling

Title:

Protease-activated receptor-2 regulates glial scar formation via JNK signaling

Creator:

Li, Tian-Zun, Liu, Qiang , Xia, Yong-Zhi, Darwazeh, Rami , and Yan, Yi

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d8572532-4def-4c66-a7eb-34910a661746
uuid:d8572532-4def-4c66-a7eb-34910a661746
doi:10.33549/physiolres.933908

Subject:

Protease-activated receptor-2, Spinal cord injury, and Jun N-terminal kinase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The study aimed to determine the effects of protease-activated receptor-2 (PAR-2) on glial scar formation after spinal cord injury (SCI) in Sprague–Dawley (SD) rats and the underlying mechanisms. Rivlin and Tator’s acute extradural clip compression injury (CCI) model of severe SCI was established in this study. Animals were divided into four groups: 1) sham group (laminectomy only); 2) model group, treated with normal saline; 3) PAR-2 inhibitor group; 4) PAR-2 activator group. Enhanced GFAP and vimentin expression were the markers of glial scar formation. To determine whether JNK was involved in the effects of PAR-2 on GFAP and vimentin expression, we administered anisomycin (a JNK activator) in the presence of PAR-2 inhibitor and SP600125 (a JNK inhibitor) in the presence of PAR-2 activator. At 1, 7, 14 and 28 day after SCI, Basso, Beattie, and Bresnahan (BBB) locomotor score test was used to assess the locomotor functional recovery; immunofluorescence and western blot analysis were used to assess the expression level of GFAP, vimentin and p-JNK. Double immunofluorescence staining with GFAP and tubulin β was used to assess the glial scar formation and the remaining neurons. Results suggested that PAR-2 is involved in glial scar formation and reduces neurons residues which can cause a further worsening in the functional outcomes after SCI via JNK signaling. Therefore, it may be effective to target PAR-2 in the treatment of SCI.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

305-316

Source:

Physiological research | 2019 Volume:68 | Number:2

Harvested from:

CDK

Metadata only:

false