Somatotropin has no effect on the quantity of guanine nucleotide binding proteins Gqalpha/G11alpha in goat adipose tissue in vivo

Title:

Somatotropin has no effect on the quantity of guanine nucleotide binding proteins Gqalpha/G11alpha in goat adipose tissue in vivo

Creator:

Krbeček, V., Hana Kovářů, Josef Škarda, Tomislav Barth, Jiří Velek, and Žižkovský, V.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d8cf9a5a-83e8-49c6-8d9b-72aa122cad12
uuid:d8cf9a5a-83e8-49c6-8d9b-72aa122cad12
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, tuková tkáň, kozy, adipose tissue, goats, samatotropin, g-proteins, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The decapeptide QLNLKEYNLV corresponding to the C-terminus of Gq/G11alpha guanine nucleotide-binding proteins (G-proteins) was synthesized by the solid-phase method and conjugated to keyhole limpet hemocyanin. The rabbits were immunized with these conjugates and an antiserum that reacted specifically with the alpha subunit of Gq/G11 proteins was used in this study. The antiserum exhibited no cross-reactivity with the alpha subunits of stimulatory (Gs) or inhibitory (Gi) G-proteins associated with adenylate cyclase. Immunoblots with the antiserum showed that it specifically recognized the Gq/G11 alpha-proteins in cholate extracts of adipose tissue membranes of goats. Treatment of young castrated male goats with bST had no effect on the quantity of Gq/G11 alpha-subunits in adipose tissue and the results thus obtained did not support the idea that the bST signal in adipose tissue is transmitted via Gq/G11 alpha-proteins., V. Krbeček, H. Kovářů, J. Škarda, T. Barth, J. Velek, V. Žižkovský., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2000 Volume:49 | Number:6

Harvested from:

CDK

Metadata only:

false