The Response of Na+/K+-ATPase of Human Erythrocytes to Green Laser Light Treatment

Title:

The Response of Na+/K+-ATPase of Human Erythrocytes to Green Laser Light Treatment

Creator:

Kaššák, P., Šikurová, L., Kvasnička, P., and Bryszewska, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:dd269137-0c4f-4e04-ae68-ecfc0cf5be1b
uuid:dd269137-0c4f-4e04-ae68-ecfc0cf5be1b

Subject:

Na+/K+-ATPase activity, Biostimulation, Erythrocyte membrane, Merocyanine 540, and Photodynamic processes

Type:

article, model:article, and TEXT

Description:

The objective of this study was to investigate the response of Na+/K+-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm-2 and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medium with 2 mM ATP for 30 min. Activity of ATPase was determined colorimetrically by measuring the colored reaction product of liberated inorganic phosphate and malachite green at 640 nm. Contribution of Na+/K+-ATPase to overall phosphate production was determined using ouabain. A positive effect of green laser light on Na+/K+-ATPase activity was observed. The dependence of enzymatically liberated inorganic phosphate on light fluence showed a linear correlation (R2=0.96, P=0.0005) for all fluences applied (9.5-63.3 J.cm-2). On the other hand, MC 540-mediated phototreatment caused a suppression of enzyme activity.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2006 Volume:55 | Number:2

Harvested from:

CDK

Metadata only:

false