Hepcidin downregulation by repeated bleeding is not mediated by soluble hemojuvelin

Title:

Hepcidin downregulation by repeated bleeding is not mediated by soluble hemojuvelin

Creator:

Jan Krijt, Fujikura, Y., Luděk Šefc, Martin Vokurka, Hlobeňová, T., and Emanuel Nečas

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e3f795f3-4d78-4c5b-8be4-500163079d07
uuid:e3f795f3-4d78-4c5b-8be4-500163079d07

Subject:

Fyziologie člověka a srovnávací fyziologie, hemoragie, hematologie, patologická fyziologie, hemorrhage, hematology, pathological physiology, hepcidin, hemojuvelin, furin, tmprss6, matriptase-2, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Hepcidin is a key regulator of iron homeostasis, while hemojuvelin is an important component of the hepcidin regulation pathway. It has been recently proposed that soluble hemojuvelin, produced from hemojuvelin by the protease furin, decreases hepcidin expression. The aim of the presented study was to examine the downregulation of hepcidin by chronic bleeding in hemojuvelin-mutant mice. Male mice with targeted disruption of the hemojuvelin gene (Hjv-/- mice) and wild-type littermates were maintained on an iron-deficient diet and subjected to weekly phlebotomies for 7 weeks. Gene expression was examined by real-time PCR. In wild type mice, repeated bleeding decreased hepcidin mRNA by two orders of magnitude. In Hjv-/- mice, basal hepcidin expression was low; however, repeated bleeding also decreased hepcidin mRNA content by an order of magnitude. Phlebotomies reduced hepatic iron overload in Hjv-/- mice by 80 %. Liver and muscle furin mRNA content was not significantly changed. No effect on hepatic Tmprss6 mRNA content was observed. Results from the study indicate that soluble hemojuvelin is not the sole factor responsible for hepcidin downregulation. In addition, the presented data suggest that, under in vivo conditions, tissue hypoxia does not transcriptionally regulate the activity of furin or TMPRSS6 proteases., J. Krijt ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:1

Harvested from:

CDK

Metadata only:

false