PsbP-induced protein conformational changes around Cl- ions in the water oxidizing center of photosystem II

Title:

PsbP-induced protein conformational changes around Cl- ions in the water oxidizing center of photosystem II

Creator:

Kondo, J. and Noguchi, T.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e5497147-4fbd-4dbc-b70e-93c41c516edc
uuid:e5497147-4fbd-4dbc-b70e-93c41c516edc
issn:0300-3604
doi:10.1007/s11099-017-0749-5

Subject:

fotosyntéza, photosynthesis, Mn4CaO5 cluster, oxygen evolution, 2, and 581

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

PsbP is an extrinsic protein of PSII having a function of Ca2+ and Cl- retention in the water-oxidizing center (WOC). In order to understand the mechanism how PsbP regulates the Cl- binding in WOC, we examined the effect of PsbP depletion on the protein structures around the Cl- sites using Fourier transform infrared (FTIR) spectroscopy. Light-induced FTIR difference spectra upon the S1-S2 transition were obtained using Cl--bound and NO3--substituted PSII membranes in the presence and absence of PsbP. A clear difference in the amide I band changes by PsbP depletion was observed between Cl--bound and NO3--substituted PSII samples, indicating that PsbP binding perturbed the protein conformations around the Cl-ion(s) in WOC. It is suggested that PsbP stabilizes the Cl- binding by regulating the dissociation constant of Cl- and/or an energy barrier of Cl- dissociation through protein conformational changes around the Cl- ion(s)., J. Kondo, T. Noguchi., and Obsahuje bibliografické odkazy

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

178-184

Source:

Photosynthetica | 2018 Volume:56 | Number:1

Harvested from:

CDK

Metadata only:

false