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Start Over Coverage 178-184

2. PsbP-induced protein conformational changes around Cl- ions in the water oxidizing center of photosystem II

Creator:
Kondo, J. and Noguchi, T.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
fotosyntéza, photosynthesis, Mn4CaO5 cluster, oxygen evolution, 2, and 581
Language:
Multiple languages
Description:
PsbP is an extrinsic protein of PSII having a function of Ca2+ and Cl- retention in the water-oxidizing center (WOC). In order to understand the mechanism how PsbP regulates the Cl- binding in WOC, we examined the effect of PsbP depletion on the protein structures around the Cl- sites using Fourier transform infrared (FTIR) spectroscopy. Light-induced FTIR difference spectra upon the S1-S2 transition were obtained using Cl--bound and NO3--substituted PSII membranes in the presence and absence of PsbP. A clear difference in the amide I band changes by PsbP depletion was observed between Cl--bound and NO3--substituted PSII samples, indicating that PsbP binding perturbed the protein conformations around the Cl-ion(s) in WOC. It is suggested that PsbP stabilizes the Cl- binding by regulating the dissociation constant of Cl- and/or an energy barrier of Cl- dissociation through protein conformational changes around the Cl- ion(s)., J. Kondo, T. Noguchi., and Obsahuje bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Ramanova mikrospektroskopie a mapování buněk a tkání

Creator:
Mojzeš, Peter, Palacký, Jan, Bauerová, Václava, and Bednárová, Lucie
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
Ramanova spektroskopie, buňky, tkáně, Raman spectroscopy, cells, tissues, mikrospektroskopie, 6, and 53
Language:
Czech and English
Description:
Raman microspectroscopy (μRS) and imaging is gradually becoming a practical tool for contactless and nondestructive analysis of microscopis objects. Due to the combination of chemical specificity of vibrational spectra and spatial resolution offered by confocal optical microscopy, μRS techniques are particularly suitable for studying chemical composition and morphology of living cells and biological tissues. Progress in experimental technniques and development of methods making the informational richness of vibrational spectra more accessible allowed expansion of μRS beyond the walls of specialized spectroscopis laboratories directly into biomedical practice., Ramanova mikrospektroskopie (μRS) a zobrazování se postupně prosazuje jako praktický nástroj pro bezkontaktní a nedestruktivní analýzu mikroskopických objektů. Díky kombinaci chemické specificity vibračních spekter a prostorového rozlišení poskytovaného konfokální optickou mikroskopií jsou techniky μRS zvlášť vhodné pro studium chemického složení a morfologie živých buněk i biologických tkání. Pokrok v experimentální technice a rozvoj metod zpřístupňujících informační bohatství vibračních spekter umožnily rozšíření μRS mimo zdi specializovaných spektroskopických laboratoří přímo do biomedicínské praxe., Peter Mojzeš, Jan Palacký, Václava Bauerová, Lucie Bednárová., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public