Akt substrate of 160 kDa dephosphorylation rate is reduced in insulin-stimulated rat skeletal muscle after acute exercise

Title:

Akt substrate of 160 kDa dephosphorylation rate is reduced in insulin-stimulated rat skeletal muscle after acute exercise

Creator:

Arias, E. B, Wang, H, and Cartee, G. D

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e5695c51-b5ff-4ebe-89e4-27e438972c6a
uuid:e5695c51-b5ff-4ebe-89e4-27e438972c6a
issn:0862-8408

Subject:

fyziologie člověka, human physiology, Insulin sensitivity, Glucose transport, TBC1D4, 14, and 612

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

Because greater Akt substrate of 160 kDa (AS160) phosphorylation has been reported in insulin-stimulated skeletal muscles without improved Akt activation several hours post-exercise, we hypothesized that prior exercise would result in attenuated AS160 dephosphorylation in insulin-stimulated rat skeletal muscle. Epitrochlearis muscles were isolated from rats that were sedentary (SED) or exercised 3 h earlier (3 h postexercise; 3hPEX). Paired muscles were incubated with [3H]-2-deoxyglucose (2-DG) without insulin or with insulin. Lysates from other insulin-stimulated muscles from SED or 3hPEX rats were evaluated using AS160Thr642 and AS160Ser588 dephosphorylation assays. Prior exercise led to greater 2-DG uptake concomitant with greater AS160Thr642 phosphorylation and a non-significant trend (P=0.087) for greater AS160Ser588. Prior exercise also reduced AS160Thr642 and AS160Ser588 dephosphorylation rates. These results support the idea that attenuated AS160 dephosphorylation may favor greater AS160 phosphorylation post-exercise., E. B. Arias, H. Wang, G. D. Cartee., and Seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2018 Volume:67 | Number:1

Harvested from:

CDK

Metadata only:

false