A newly developed SGB buffer greatly enhances energy transfer efficiency from phycobilisomes to photosystem II in cyanobacteria in vitro

Title:

A newly developed SGB buffer greatly enhances energy transfer efficiency from phycobilisomes to photosystem II in cyanobacteria in vitro

Creator:

Chen, L. P., Wang, Q. X., and Ma, W. M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e7e29297-2112-4a53-87c7-31441ea2c7f1
uuid:e7e29297-2112-4a53-87c7-31441ea2c7f1
issn:0300-3604
doi:10.1007/s11099-013-0018-1

Subject:

botanika, botany, energy transfer efficiency, photosystem II, phycobilisome, SGB buffer, Synechocystis sp. PCC 6803, thylakoid membrane, 2, and 58

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The transfer of light energy from phycobilisomes (PBS) to photosystem II (PSII) reaction centers is vital for photosynthesis in cyanobacteria and red algae. To investigate the relationship between PBS and PSII and to optimize the energy transfer efficiency from PBS to PSII, isolation of the PBS-PSII supercomplex is necessary. SPC (sucrose/phosphate/citrate) is a conventional buffer for isolating PBS-PSII supercomplex in cyanobacteria. However, the energy transfer occurring in the supercomplex is poor. Here, we developed a new buffer named SGB by adding 1M glycinebetaine and additional sucrose to SPC buffer. Compared to SPC, the newly developed SGB buffer greatly enhanced the associated populations of PBS with thylakoid membranes and PSII and further improved the energy transfer efficiency from PBS to PSII reaction centers in cyanobacteria in vitro. Therefore, we conclude that SGB is an excellent buffer for isolating the PBS-PSII supercomplex and for enhancing the energy transfer efficiency from PBS to PSII reaction centers in cyanobacteria in vitro., L. P. Chen, Q. X. Wang, W. M. Ma., and Obsahuje bibliografii

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

215-220

Source:

Photosynthetica | 2013 Volume:51 | Number:2

Harvested from:

CDK

Metadata only:

false