Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice

Title:

Drugs elevating extracellular adenosine administered in vivo induce serum colony-stimulating activity and interleukin-6 in mice

Creator:

Lenka Weiterová, Michal Hofer, Milan Pospíšil, Vladimír Znojil, and Denisa Štreitová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:eb489dd0-c921-4066-a5ba-020fb7b4991b
uuid:eb489dd0-c921-4066-a5ba-020fb7b4991b
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, biofyzika, fyziologie živočichů, adenosin, biophysics, animal physiology, adenosine, granulopoeza, faktor stimulující kolonie granulocytů (G-CSF), interleukin-6 (IL-6), granulopoiesis, granulocyte colony-stimulating factor (G-CSF), interleukin 6 (IL-6), GM-CFC, serum colony-stimulating activity, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stimulating activity (CSA) of blood serum of mice treated with DP+AMP, G-CSF or all these drugs in combination, i.e. the ability of the sera to stimulate the growth of GM-CFC colonies, was assayed in vitro. Furthermore, the concentration of GM-CSF and IL-6 in the sera was determined. Administration of DP+AMP was found to enhance significantly serum CSA at all time intervals of serum sampling including 24 h after the last injection of the tested drugs. Additive effects of DP+AMP and G-CSF on serum CSA were noted at early intervals after administration of the drugs. Furthermore, IL-6 levels were significantly elevated in the sera of mice which were administered DP+AMP either alone or in combination with G-CSF. Our results show that the effects of DP+AMP are indirect, mediated through the induction of some cytokine(s) and/or growth factor(s) and that extracellular adenosine can act in cooperation with G-CSF. These findings contribute to the further elucidation of the role of adenosine in hematopoiesis., L. Weiterová, M. Hofer, M. Pospíšil, V. Znojil, D. Štreitová., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:4

Harvested from:

CDK

Metadata only:

false