Selective inhibition of NF-κB and surfactant therapy in experimental meconium-induced lung injury

Title:

Selective inhibition of NF-κB and surfactant therapy in experimental meconium-induced lung injury

Creator:

Kopincova, J., Mikolka, P., Kolomaznik, M., Kosutova, P, Andrea Čalkovská, and Daniela Mokrá

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ee7102e8-42e8-4b50-912a-6673bef004a8
uuid:ee7102e8-42e8-4b50-912a-6673bef004a8
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, záněty, inflammations, meconium-induced lung injury, surfactant treatment, NF-κB, IKK-NBD, 14, and 612

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Meconium aspiration syndrome (MAS) in newborns is characterized mainly by respiratory failure due to surfactant dysfunction and inflammation. Previous meta-analyses did not prove any effect of exogenous surfactant treatment nor glucocorticoid administration on final outcome of children with MAS despite oxygenation improvement. As we supposed there is the need to intervene in both these fields simultaneously, we evaluated therapeutic effect of combination of exogenous surfactant and selective inhibitor of NF-κB (IKK-NBD peptide). Young New Zealand rabbits were instilled by meconium suspension and treated by surfactant alone or surfactant in combination with IKK-NBD, and oxygen-ventilated for 5 h. PaO2/FiO2, oxygenation index, oxygen saturation and ventilation efficiency index were evaluated every hour; post mortem, total and differential leukocyte counts were investigated in bronchoalveolar lavage fluid (BALF) and inflammatory, oxidative and apoptotic markers were assessed in lung tissue homogenates. Exogenous surfactant combined with IKK-NBD improved oxygenation, reduced neutrophil count in BALF and levels of IL-1β, IL-6, p38 MAPK and caspase 3 in comparison with surfactant-only therapy. It seems that inhibition of inflammation may be strong supporting factor in surfactant treatment of MAS., J. Kopincova, P. Mikolka, M. Kolomaznik, P. Kosutova, A. Calkovska, D. Mokra., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2017 Volume:66 | Number:Suppl 2

Harvested from:

CDK

Metadata only:

false