Transient upregulation of protein kinase C in pressure-overloaded neonatal rat myocardium

Title:

Transient upregulation of protein kinase C in pressure-overloaded neonatal rat myocardium

Creator:

Blanka Hamplová, František Novák, František Kolář, and Olga Nováková

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:efa3472d-384c-4311-aae6-44220deee74f
uuid:efa3472d-384c-4311-aae6-44220deee74f

Subject:

Fyziologie člověka a srovnávací fyziologie, proteinové kinázy, protein kinases, protein kinase C, pressure overload, postnatal development, rat myocardium, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Protein kinase C (PKC) appears to play a significant role in the signal transduction of cardiac growth and development. The aim of this study was to determine changes in the total PKC activity and the expression of PKC isoforms α, δ and ε in the rat heart that was affected by pressure overload imposed at postnatal day (d) 2. Three groups of Wistar rats were employed for the experiment: rats submitted to the abdominal aortic constriction (AC), sham-operated controls (SO) and intact controls. Animals were sacrificed at d2, d3, d5 and d10. The total PKC activity was measured by the incorporation of 32P into histone IIIS and the expression of PKC was analyzed by immunoblotting in the homogenate of the left ventricular myocardium and in the cytosolic, membrane-enriched (105 × g) and nuclear-cytoskeletalmyofilament- enriched (103 × g) fractions. We observed the significant transient increase in both the total PKC activity and the expression of all isoforms at d5 (the 3rd day after the operation) in the cardiac homogenate of AC rats as compared with SO animals. Aortic constriction did not significantly affect the distribution of activity and isoform abundance among individual cellular fractions except for PKCδ, which increased significantly at d10 in the cytosolic fraction at the expense of the membraneenriched fraction. It is concluded that PKCα, PKCδ and PKCε undergo transient upregulation associated with the accelerated cardiac growth induced by pressure overload imposed in the very early postnatal period., B. Hamplová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:1

Harvested from:

CDK

Metadata only:

false