Involvement of phospholipids in the mechanism of insulin action in HEPG2 cells

Title:

Involvement of phospholipids in the mechanism of insulin action in HEPG2 cells

Creator:

Novotná, R., De Vito, P., Currado, L., Luly, P., and Baldini, P. M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:f17a9773-0009-4f82-8067-f395fb4b9fd5
uuid:f17a9773-0009-4f82-8067-f395fb4b9fd5
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, inzulin, insulin, phosphatidic acid, diacylglycerol, phospholipases, HEPG2 cells, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The mechanism of action by which insulin increases phosphatidic acid (PA) and diacylglycerol (DAG) levels was investigated in cultured hepatoma cells (HEPG2). Insulin stimulated phosphatidylcholine (PC) and phosphatidyl-inositol (PI) degradation through the activation of specific phospholipases C (PLC). The DAG increase appears to be biphasic. The early DAG production seems to be due to PI breakdown, probably through phosphatidyl-inositol-3-kinase (PI3K) involvement, whereas the delayed DAG increase is derived directly from the PC-PLC activity. The absence of phospholipase D (PLD) involvement was confirmed by the lack of PC-derived phosphatidylethanol production. Experiments performed in the presence of R59022, an inhibitor of DAG-kinase, indicated that PA release is the result of the DAG-kinase activity on the DAG produced in the early phase of insulin action., R. Novotná, P. de Vito, L. Currado, P. Luly, P. M. Baldini., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2003 Volume:52 | Number:4

Harvested from:

CDK

Metadata only:

false