Changes in the composition of fatty acids and lipofuscin-like pigments during development of rat heart

Title:

Changes in the composition of fatty acids and lipofuscin-like pigments during development of rat heart

Creator:

Wilhelm, J., Joško Ivica, Veselská, Z., Jiří Uhlík, and Luděk Vajner

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fa7cbfd8-f4c9-47fe-b037-a6d7bf6bb169
uuid:fa7cbfd8-f4c9-47fe-b037-a6d7bf6bb169
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, mastné kyseliny, volné radikály, srdce, fatty acids, free radicals, heart, development, lipofuscinlike pigments, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Postnatal heart development is characterized by critical periods of heart remodeling. In order to characterize the changes in the lipophilic fraction induced by free radicals, fatty acids and t heir oxidized products, lipofuscin -like pigments (LFP), were investigated. Fatty acids were analyz ed by gas chromatography and LFP were studied by fluorescence techniques. A fluorophore characterized by spectral methods was further resolved by HPLC. Major changes in the composition of fatty acids occurred immediately after birth and then during maturation. Fluorescence of LFP changed markedly on postnatal days 1, 4, 8, and 14, and differed from the adult animals. LFP comprise several fluorophores that were present since fetal state till adulthood. No new major fluorophores were formed during development, just the abundances of individual fluorophores have been modulated which produced changes in the shape of the spectral arrays. HPLC resolved the fluorophore with excitation maximum at 360 nm and emission maximum at 410 nm. New chromatographically distinct species appeared immediately on postnatal day 1, and then on days 30 and 60. Consumption of polyunsaturated fatty acids immediately after birth and subseque nt formation of LFP suggests that oxidative stress is involved in normal heart development., J. Wilhelm, J. Ivica, Z. Veselská, J. Uhlík, L. Vajner., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:5

Harvested from:

CDK

Metadata only:

false