Negative effects of P-buffering and pH on photosynthetic activity of planktonic desmid species

Title:

Negative effects of P-buffering and pH on photosynthetic activity of planktonic desmid species

Creator:

Spijkerman, E., Garcia-Mendoza, E., Matthijs, H. C. P., van Hunnik, E., and Coesel, P. F. M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fab2618d-65f7-4d96-8c94-b8719b7ef007
uuid:fab2618d-65f7-4d96-8c94-b8719b7ef007
issn:0300-3604
doi:10.1023/B:PHOT.0000040569.17719.2a

Subject:

Desmidiaceae, high irradiance stress, inorganic carbon limitation, Na+, non-photochemical quenching, photosystem 2, pH-stress, Staurastrum, and Staurodesmus

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The photosynthetic activities of three planktonic desmid species (Staurastrum brachiatum, Staurodesmus cuspidatus var. curvatus, and Staurastrum chaetoceras) were compared after adaptation to medium enriched with either a 20 mM Na+-phosphate (P) or HEPES buffer. Incubations up to 2 d were carried out at pH 6 or 8 under normal air or air enriched with 5 % CO2. Gross maximum photosynthetic rate (Pmax) and growth rate were decreased in both S. brachiatum and Std. cuspidatus at higher pH when using the HEPES buffer and this effect was independent of CO2 concentration, indicating that pH had an inhibitory effect on photosynthesis and growth in these species. The P-buffer at pH 8 caused a large decrease in Pmax and quantum yield for charge separation in photosystem 2 (PS2), compared to HEPES-buffered algae. This effect was very large in both S. brachiatum and Std. cuspidatus, two species characteristic of soft water lakes, but also significant in S. chaetoceras, a species dominant in eutrophic, hard water lakes. The decreased Pmax in P-buffer could not be related to a significant increase in cellular P content known to be responsible for inhibition in isolated chloroplasts. Experiments at pH 6 and 8 showed that two conditions, high pH and high Na+ concentration, both contributed to the decreased Pmax and quantum yield in the desmids. Effects of a P-buffer were less pronounced by using K+-P buffer. The use of P-buffer at pH 8 possibly resulted in high irradiance stress in all species, indicated by damage in the PS2 core complex. In the soft water species pH 8 resulted in increased non-photochemical quenching together with a high de-epoxidation state of the xanthophyll cycle pigments. and E. Spijkerman ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

49-57

Source:

Photosynthetica | 2004 Volume:42 | Number:1

Harvested from:

CDK

Metadata only:

false