Effect of sophoridine on Ca2+ induced Ca2+ release during heart failure

Title:

Effect of sophoridine on Ca2+ induced Ca2+ release during heart failure

Creator:

Hu, S.-T., Shen, Y.-F., Gong, J.-M., and Yang, Y.-J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fd3d3970-ebda-4f13-a48a-5831ab3613a3
uuid:fd3d3970-ebda-4f13-a48a-5831ab3613a3
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, srdeční selhání, heart failure, sophoridine, Ca2+ induced Ca2+ release, l-type Ca2+ channel, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Sophoridine is a type of alkaloid extract derived from the Chinese herb Sophora flavescens Ait (kushen) and possess a variety of pharmacological effects including anti- inflammation, anti - anaphylaxis, anti - cancer, anti - arrhythmic and so on. However, the effect of sophoridine on heart failure has not been known yet. In this study, the effect of sophoridine on heart failure was investigated using Sprague -Dawley (SD) rat model of chronic heart failure. Morphological results showed that in medium and high dose group, myofilaments were arranged orderly and closely, intermyofibrillar ly sis disappeared and mitochondria contained tightly packed cristae compared with heart failure group. We investigated the Ca 2+ induced Ca 2+ transients and assessed the expression of ryanodine receptor (RyR2) and L-type Ca 2+ channel (dihydropyridine receptor, DHPR). We found that the cytosolic Ca 2+ transients were markedly increas ed in amplitude in medium ( ΔF/F 0 =43.33±1.92 ) and high dose groups ( ΔF/F 0 =47.21 ±1.25 ) compared with heart failure group ( ΔF/F 0 =16.7±1.29, P <0.0 1), Moreover, we demonstrated that the expression of cardiac DHPR was significantly increased in medium - and high dose -group compared with heart failure rats. Our results suggest that sophoridine could improve heart failure by ameliorating cardiac Ca 2+ induced Ca 2+ transients, and that thi s amelioration is associated with upregulation of DHPR., S.-T. Hu, Y.-F. Shen, J.-M. Gong, Y.-J. Yang., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2016 Volume:65 | Number:1

Harvested from:

CDK

Metadata only:

false