Mass spectrometry analyses of rat 2b myosin heavy chain isoform

Title:

Mass spectrometry analyses of rat 2b myosin heavy chain isoform

Creator:

Jitka Žurmanová, Daniela Horníková, František Půta, Petr Novák, Jan Říčný, and Tomáš Soukup

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fd402cb0-9a80-4130-8907-207b81bd8a43
uuid:fd402cb0-9a80-4130-8907-207b81bd8a43
issn:0862-8408

Subject:

Obecná zoologie, fyziologie živočichů, mikrobiologie, spektrometrie, animal physiology, microbiology, spectrometry, rat muscle myosin, myosin heavy chain 2b isoform, MALDI TOF mass spectrometry, ESI LC/MS/MS mass spectrometry, 2, and 591

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

We have separated 2b myosin heavy chain (MyHC) isoform from the rat extensor digitorum longus muscle by SDS-PAGE and analyzed it by two subsequent mass spectrometry techniques. After tryptic digestion, the obtained peptides were identified by Matrix-Assisted Laser Desorption/Ionisation reflectron Time of Flight mass spectrometry (MALDI-TOF MS) and sequenced by Liquid chromatography tandem mass spectrometry (ESI LC/MS/MS). The analyzed peptides proportionally covered 30 % of the 2b MyHC isoform sequence. The results suggest that the primary structure is identical with the highest probability to a NCBI database record ref|NP_062198.1|, representing the last updated record of rat 2b isoform. Nonetheless, four peptides carrying amino acid substitution(s) in comparison with the NCBI database record were identified., J. Žurmanová, D. Maláčová, F. Půta, P. Novák, J. Říčný, T. Soukup., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:5

Harvested from:

CDK

Metadata only:

false