Effect of nitric oxide release from NOR-3 on urea synthesis, viability and oxygen consumption of rat hepatocyte cultures

Title:

Effect of nitric oxide release from NOR-3 on urea synthesis, viability and oxygen consumption of rat hepatocyte cultures

Creator:

Chimenti, R., Martino, G., Mazzulla, S., and Sesti, S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fd4a26a2-a0d3-4d0f-a3fb-bc2eb0e9b220
uuid:fd4a26a2-a0d3-4d0f-a3fb-bc2eb0e9b220
issn:0862-8408

Subject:

Buněčná biologie. Cytologie, fyziologie, oxid dusnatý, jaterní buňky, physiology, nitric oxide, liver cells, 4,5 diamminofluorescein diacetate, nitric oxide donor, rat hepatocytes, 2, and 576

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

As nitric oxide is considered a mediator of liver oxid ative metabolism during sepsis, we studied the effects of exogenous nitric oxide, produced by NO-donor, (±)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (NOR-3), on cell viability, urea biosynthesis and oxygen consumption in rat hepatocyte cultures. Nitric oxide release from NOR-3 was studied using 4,5-diaminofluorescein diacetate. Urea levels were measured by the spectrophotometric method. Cell viability was determined by the MTT test and trypan blue exclusion test, whereas oxygen consumption was measured by a polarographic technique. After 2 h treatment, NOR-3 induced an increase in the levels of nitric oxide. After 2 h of treatment and 24 h after the end of the treatment with NOR-3, both cell viability and urea synthesis were significantly reduced in comparison to the controls for NOR-3 concentrations equal to or greater than 50 μM. A reduction in oxygen consumption was observed in hepatocytes after 40 min treatment with 100 μM NOR-3, even if the cell viability was unchanged. Reduction of oxygen consumption is an early indicator of the metabolic alterations in hepatocytes exposed to nitric oxide. These findings suggest that nitric oxide accumulation acts on hepatocyte cultures inducing cell death and reduction of urea synthesis after 2 hours., R. Chimenti, G. Martino, S. Mazzulla, S. Sesti., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:4

Harvested from:

CDK

Metadata only:

false