Various AKIP1 expression levels affect its subcellular localization but have no effect on NF-κB activation

Title:

Various AKIP1 expression levels affect its subcellular localization but have no effect on NF-κB activation

Creator:

Keprová, A., Kořínková, L. , Křížová, I. , Hadravová, R., Kaufman, F., Pichová, I. , Ruml, T., and Rumlová, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ffbf26e8-0eba-4382-a6f2-eea284a894c1
uuid:ffbf26e8-0eba-4382-a6f2-eea284a894c1
doi:10.33549/physiolres.933961

Subject:

AKIP1, BCA3, protein aggregation, NF-kappa B, and transcription regulation

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

A-kinase interacting protein 1 (AKIP1) has been shown to interact with a broad range of proteins involved in various cellular processes, including apoptosis, tumorigenesis, and oxidative stress suggesting it might have multiple cellular functions. In this study, we used an epitope-tagged AKIP1 and by combination of immunochemical approaches, microscopic methods and reporter assays we studied its properties. Here, we show that various levels of AKIP1 overexpression in HEK-293 cells affected not only its subcellular localization but also resulted in aggregation. While highly expressed AKIP1 accumulated in electron-dense aggregates both in the nucleus and cytosol, low expression of AKIP1 resulted in its localization within the nucleus as a free, non-aggregated protein. Even though AKIP1 was shown to interact with p65 subunit of NF-κB and activate this transcription factor, we did not observe any effect on NF-κB activation regardless of various AKIP1 expression level.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

431-443

Source:

Physiological research | 2019 Volume:68 | Number:3

Harvested from:

CDK

Metadata only:

false