The macrophage cell-line J774.E1 and Leishmania m. mexicana infection was used to investigate the uptake of liposomes, which differed in their bulk phospholipid: ester- or ether-analogue of phosphatydilcholine (PC). The receptor-mediated uptake of both species of liposomes, containing native or acetylated LDL as ligands was also evaluated. Uninfected and infected J774.E1 cell-line accumulated more ester- and ether-liposomes alone than mixed type (50:50, ester/ether). The utilization was significantly enhanced when both types of liposomes contained native LDL. The highest uptake was recorded for liposomes bearing acetylated LDL by infected J774.E1 cells. Accumulation of ester- and ether-liposomes with the same ligand was not markedly affected by different chemical nature of PC. Finally, ether-liposomes alone possessed certain activity against Leishmania m. mexicana amastigotes. The results presented here demonstrated the usefulness of ether-liposomes with specific ligands in site-specific delivery of antileishmanial compounds in vitro.
The review is devoted to the outstanding contributions to the path of carbon in photosynthesis by Professor Emeritus Andrew A. Benson, on the occasion of his death at the age of 97, on January 16, 2015. Benson is the legendary co-discoverer of the photosynthetic reductive pentose phosphate cycle, known to every student of photosynthesis as the Benson-Bassham-Calvin cycle. This pathway evolved into the dominant assimilation mechanism for atmospheric carbon into metabolites. The fundamental ecological and biochemical optimization and evolutionary stability of this mechanism unfolded elegantly in Benson’s hands, as he was the first to recognize the building blocks for the synthesis of essential organic compounds that satisfy the energetic needs and demands of most life forms. Photosynthetic carbon metabolism together with other energy and oxidative reactions and secondary biosynthetic processes are critical for the formation of organic matter; and, thereby, the Benson-Bassham-Calvin cycle ensures maintenance of the biosphere., K. Biel, I. Fomina., and Obsahuje bibliografii
Diurnal patterns of canopy net photosynthetic rates (jPií), evapotranspiration (£) and
water use efBciency (WŤJE = P^^E) of field grown irrigated wheat (Triticum
aestivum L. cv. WH 147) were studied fi'om tillering to maturity at Haryana
Agricultural University Farm, Hisar, India. Hie maximum photosynthetic rates
(^N, max) increased fi'om tillering to anthesis and declined at a rapid rate thereaňer till
maturity. Relatively high £'mav but low WUE„ax were recorded at tillering, milk ripe
and dough stages of growth. Diumally foUowed die course of photosynthetícaUy
active radiation (PAR) fiom tillering to wateiy ripeness but it declined at high PAR at
latter reproductive stages of growth. E was curvilinearly related to air temperature.
There was a lag of about 2 h between ^N, „lax (12.00 h) and E„,ax (14.00 h) during
the day. WUE was high fi-om 07.30 to 10.00 h, depressed during midday and a
slightly increased fi^om 15.00 to 16.00 h.Diurnal patterns of canopy net photosynthetic rates (jPií), evapotranspiration (£) and water use efBciency (WŤJE = P^^E) of field grown irrigated wheat (Triticum aestivum L. cv. WH 147) were studied fi'om tillering to maturity at Haryana Agricultural University Farm, Hisar, India. Hie maximum photosynthetic rates (^N, max) increased fi'om tillering to anthesis and declined at a rapid rate thereaňer till maturity. Relatively high £'mav but low WUE„ax were recorded at tillering, milk ripe and dough stages of growth. Diumally foUowed die course of photosynthetícaUy active radiation (PAR) fiom tillering to wateiy ripeness but it declined at high PAR at latter reproductive stages of growth. E was curvilinearly related to air temperature. There was a lag of about 2 h between ^N, „lax (12.00 h) and E„,ax (14.00 h) during the day. WUE was high fi-om 07.30 to 10.00 h, depressed during midday and a slightly increased fi^om 15.00 to 16.00 h.
We determined the surface-associated proteolytic activity in three Entamoeba histolytica Schaudinn, 1903 strains (monoxenic HM1, axenic HM1, and HK9) of known virulence and its relationship with collagenase activity. Both activities were also determined in axenic HM1 amoebae trophozoites which were sensitive and resistant to complement-mediated lysis. Surface proteolytic activity was determined in glutaraldehyde-fixcd E. histolytica trophozoites, which degraded the insoluble substrate, hide powder azure, and cleaved the human immunoglobulin G heavy chain in a time-dependent fashion, at neutral pH, in presence of 2-mercaptoethanol as cysteine protcase activator. Surface proteolytic activity was strain dependent: monoxenic HM1 > axenic IIM1 > axenic HK9. This activity correlated with collagenolytic activity (p < 0.05). Acquisition of resistance to complement-mediated lysis by axenic HM1 strain did not modify either surface proteases or collagenase expression. Our results suggest that this surface proteolytic activity could be used as an in vitro virulence marker for E. histolytica.
Jaderná fúze představuje pravděpodobnou budoucnost energetiky. V současnosti neexistuje jiný energetický zdroj, který by dokázal nahradit spalování fosilních paliv a štěpnou jadernou energetiku. Jaderná fúze je přitom bezpečný, ekologický a prakticky nevyčerpatelný zdroj energie. Parametry plazmatu potřebné k energetickému využití fúze popisuje jednoduchý vzorec - Lawsonovo kritérium., Nuclear fusion is the probable future of energy. Currently, there is no other energy source that could replace fossil fuels and nuclear fission energy. Nuclear fusion is also absolutely safe, highly ecological and especially, from a human point of view an inexhaustible source of energy, The link between the plasma experiment and the fusion energy recovery is represented by a simple formula - the Lawson criterion., Slavomír Entler., and Obsahuje seznam literatury
Effects of ultraviolet-B (UV-B) irradiation on ultrastructure, total cellular protein, and PS2 proteins D1 and D2 of Synechococcus sp. PCC 7942 cells was studied. The scanning electron micrographs showed UV-B radiation induced bending of the cells. The transmission electron micrographs revealed disorganization and shift in thylakoid lamellar structure to one side of the cell. The cellular phycocyanin/chlorophyll ratio decreased with increasing UV-B treatment and due to this the colour of cells turned light-green. No apparent change in total cellular proteins was evident, but the contents of two major proteins of PS2, D1 and D2, showed decline due to UV-B irradiation, although to different extent. and Sanjay Chauhan, Ritu Pandey, Gauri S. Singhal.