A comparative study of the utilization of ether- and ester-linked phospholipid-containing liposomes by J774.E1 macrophage cell-line infected with Leishmania mexicana mexicana amastigotes

Title:

A comparative study of the utilization of ether- and ester-linked phospholipid-containing liposomes by J774.E1 macrophage cell-line infected with Leishmania mexicana mexicana amastigotes

Creator:

Hrčková, G., Shah, A., Hart, D.T., and Halton, D.W.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bc08567e-fa7b-4e22-b817-8d03ceca4c0a
uuid:bc08567e-fa7b-4e22-b817-8d03ceca4c0a
urnnbn:urn:nbn:cz:aba007-000di4

Subject:

Leishmania m. mexicana, ester-, ether-linked phosphatydilcholine, igands (native LDL, acetylated LDL), and liposome uptake

Type:

model:internalpart and TEXT

Format:

print

Description:

The macrophage cell-line J774.E1 and Leishmania m. mexicana infection was used to investigate the uptake of liposomes, which differed in their bulk phospholipid: ester- or ether-analogue of phosphatydilcholine (PC). The receptor-mediated uptake of both species of liposomes, containing native or acetylated LDL as ligands was also evaluated. Uninfected and infected J774.E1 cell-line accumulated more ester- and ether-liposomes alone than mixed type (50:50, ester/ether). The utilization was significantly enhanced when both types of liposomes contained native LDL. The highest uptake was recorded for liposomes bearing acetylated LDL by infected J774.E1 cells. Accumulation of ester- and ether-liposomes with the same ligand was not markedly affected by different chemical nature of PC. Finally, ether-liposomes alone possessed certain activity against Leishmania m. mexicana amastigotes. The results presented here demonstrated the usefulness of ether-liposomes with specific ligands in site-specific delivery of antileishmanial compounds in vitro.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

161-167 and 3-9

Source:

Folia parasitologica | 1994 Volume:41 | Number:3

Harvested from:

CDK

Metadata only:

false