A variety of conditions of cryopreservation were evaluated in order to define a single procedure for freezing the amebae of pathogenic Naegleria and Acanthamoeba. The average best conditions for freezing the three species studied were; lxlO1’ exponentially growing amebae/ml of freezing medium consisting of 12% dimethylsulfoxide, 20% heat-inactivated bovine calf serum, 4% glucose, in Mix ameba medium; 30 min equilibration at 23” С (room temperature), followed by 60 min at -20° C, with storage at -70° C. Under these conditions viability after 1 month of freezing was 53% for Acanthamoeba castellami, 64% for Naegleria fowleri, and 66% fot Naegleria australiensis. After 12 months of freezing, viability was 39% for A. castellami, 47% for N. fowleri, and 53% for N. auslraliensis.
The interest in folk architecture dates back to the second half of 19th century. The Czechoslavonic Ethnographic Exhibition played an important role, but most projects of preservation of architecture were not carried out. The law from 1958 codified the preservation of settlement wholes; many individual folk buildings were to be preserved as well. Ony after 1989 a positive approach of state authorities towards folk architecture became visible. As early as in 1990, 20 preservation zones were declared in southern Bohemia; in 1995 60 new zones in the whole area of the republic were declared. Besides the approach reffering preservation of individual outstanding objects in their original localities, the method of conservation of some monuments by their transfer to the specialized open-air museums was implemented as well. The largest and the oldest one is the open-air museum in Rožnov pod Radhoštěm.opened in 1925. Other smaller museums of this kind came into being as late as in 1960s and 1970s (in Přerov nad Labem, Vysočina near Hlinsko, Třebíz near Slaný, Kouřim, Strážnice (south-eastern Moravia), Zubrnice (northern Bohemia), Rymice near Kroměříž, and others. Several similar museums are in the state of preparation (Chanovice and Velhartice near Klatovy, Vysoký Chlumec near Příbram). The present legislature gives several possibilities of preservation of folk architecture. The implementation of preservation plans is supported by state subsidies, various local as well as international programmes. and Anglický abstrakt s šifrou (št) je uveden na str. 199-200.
The article provides information on parental leave taken by fathers in the Czech Republic in comparison with the situation in other European countries. Above all, a concrete assessment based on the experience of families in which men have already taken advantage of parental leave are described; only briefly are mentioned attitudes of Czech parents generally towards parental leave taken by fathers. It introduces life-style of Czech families with fathers using parental leave on the basis of conducted qualitative research. The main stress is put on the actors´ point of view, which is presented in relation with broader social context, especially in the field of gender roles and reconciliation of family and work life. As an example, one case study was presented to point out the trends of new fatherhood and life-style of the families where father is taking parental leave. It has been found that families in which fathers have used parental leave for some period of time appreciate the experience on the whole.