Cryopreservation of pathogenic free-living amebae

Title:

Cryopreservation of pathogenic free-living amebae

Creator:

John, D.T., Eddy, P.L., and John, R.A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:03a8c89a-ac61-489d-a895-89e036e119cc
uuid:03a8c89a-ac61-489d-a895-89e036e119cc
urnnbn:urn:nbn:cz:aba007-000dht

Subject:

cryopreservation, amebae, Naegleria, and Acanthamoeba

Type:

model:internalpart and TEXT

Format:

print

Description:

A variety of conditions of cryopreservation were evaluated in order to define a single procedure for freezing the amebae of pathogenic Naegleria and Acanthamoeba. The average best conditions for freezing the three species studied were; lxlO1’ exponentially growing amebae/ml of freezing medium consisting of 12% dimethylsulfoxide, 20% heat-inactivated bovine calf serum, 4% glucose, in Mix ameba medium; 30 min equilibration at 23” С (room temperature), followed by 60 min at -20° C, with storage at -70° C. Under these conditions viability after 1 month of freezing was 53% for Acanthamoeba castellami, 64% for Naegleria fowleri, and 66% fot Naegleria australiensis. After 12 months of freezing, viability was 39% for A. castellami, 47% for N. fowleri, and 53% for N. auslraliensis.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

110-114 and 32-36

Source:

Folia parasitologica | 1994 Volume:41 | Number:2

Harvested from:

CDK

Metadata only:

false