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Start Over Creator Šebeková, K.

2. Markers of oxidative stress and antioxidant status in the plasma, urine and saliva of healthy mice

Creator:
Gyurászová, M. , Kovalčíková, A. , Janšáková, K. , Šebeková, K., Celec, P., and Tóthová, Ľ.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
antioxidant status, sex difference, biomarker, saliva, and urine
Language:
English
Description:
Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, healthy mice and to identify some sources of variability. Samples were obtained from 41 female and 37 male adult, healthy mice of the CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges of TBARS (thiobarbituric acid reactive substances), AOPP (advanced oxidation protein products), fructosamine, GSH/GSSG (reduced and oxidized glutathione) ratio, TAC (total antioxidant capacity), and FRAP (ferric reducing antioxidant power) were measured in plasma and urine, and TBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standard spectrophotometric and fluorometric methods. Salivary GSH/GSSG and urinary AOPP were higher in females. Urinary fructosamine, GSH/GSSG and FRAP were higher in males. Urinary TAC and FRAP negatively correlated with age, and urinary GSH/GSSG positively correlated with weight. We determined that urine and saliva can be obtained non-invasively from mice, in sufficient amounts for reliable oxidative status assessment. Further studies are needed to uncover whether these biofluids reflect systemic oxidative status in diseases.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

3. Rooibos Tea (Aspalathus linearis) Partially Prevents Oxidative Stress in Streptozotocin-Induced Diabetic Rats

Creator:
O., Vančová, O., Božek, P., Čársky, J., Šebeková, K., Boor, P., Nakano, M., and Greksák, M.
Type:
article, model:article, and TEXT
Subject:
Rooibos tea, Aspalathus linearis, Oxidative stress, and Streptozotocin-induced diabetes
Language:
English
Description:
The aim of this study was to investigate the effects of rooibos tea as a natural source of a wide scale of antioxidants on the prevention and treatment of oxidative stress in streptozotocin-induced diabetic rats. Expected significant changes of biochemical parameters characteristic for experimental diabetic state were found in plasma and tissues eight weeks after single dose streptozotocin application. Administration of aqueous and alkaline extracts of rooibos tea (or N-acetyl-L-cysteine for comparison) to diabetic rats did not affect markers of the diabetic status (glucose, glycated hemoglobin and fructosamine). Besides the parameters characterizing hepatotoxic effect of streptozotocin, rooibos tea significantly lowered advanced glycation end-products (AGEs) and malondialdehyde (MDA) in the plasma and in different tissues of diabetic rats, particularly MDA concentration in the lens. From these results we can conclude that antioxidant compounds in rooibos tea partially prevent oxidative stress and they are effective in both hydrophobic and hydrophilic biological systems. Therefore, rooibos tea as a commonly used beverage can be recommended as an excellent adjuvant support for the prevention and therapy of diabetic vascular complications, particularly for protecting ocular membrane systems against their peroxidation by reactive oxygen species.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public