To this day five Gobio gobio subspecies in northern, western and central Anatolia, in Turkey have been classified. Some subspecies were described based on 2-4 specimens by B a t t a l g i l (1942, 1944), L a d i g e s (1960), B ă n ă r e s c u & N a l b a t (1973), B ă n ă r e s c u (1992), B ă n ă r e s c u (1999). In this study, Gobio gobio specimens from the type localities of various running waters and lakes of norht, norhwest and central Anatolia were collected and ANOVA (analysis of variance), cluster analysis and canonical discriminant analysis were applied to fifty variables (eight meristic and forty-two metric characters derived from thirty-four measured or counted variables) to show the main differences between them. From these statistical analyses, Gobio gobio specimens were placed in one of three groups: G1 Beyşehir Lake; G2 Sakarya River Basin, Gerede and Melendiz Stream; G3 İnsuyu Stream, southwards from Tuz Lake. Measurements Standard length/Head height (SL/hH), Head length/Head height (C/hH), Standard length/Head length (SL/C), Predorsal distance/Postdorsal distance (pD/poD), Standard length/Postdorsal distance (SL/poD) and Standard length/Predorsal distance (SL/pD) were important characteristics for distinguishing the populations of Gobio gobio.