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2. Je libo játra nebo mozeček? Několik zastavení s motolicemi
- Creator:
- Horák, Petr, Mikeš, Libor, and Kašný, Martin
- Type:
- article and TEXT
- Language:
- Czech
- Description:
- Motolice parazitující v játrech a centrální nervové soustavě lidí a zvířat jsou stále hojně rozšířeny na celé Zemi. Mohou působit nezanedbatelné zdravotní i ekonomické problémy, a proto jsou možnosti jejich potlačení trvale žhavými náměty mnoha vědeckých projektů. Kromě poznání ontogenetického vývoje, způsobu přenosu a patogenity těchto parazitů je v popředí zájmu i studium jejich biologicky aktivních molekul (např. histolytické či metabolické enzymy, dominantní antigeny), jejichž poznání může vést ke spolehlivější diagnostice, efektivnější léčbě a prevenci onemocnění vyvolaných motolicemi. and Flukes parasitizing the liver and central nervous systems of humans and animals are still widespread all over the world. They can cause serious health and economic losses, so the means of their eradication are a hot topic for many research projects. Besides a description of trematode development, transmission and pathogenicity, there is a study of biologically active parasite molecules (e.g. histolytic and metabolic enzymes, immunodominant antigens). A characterization of these molecules may lead to a reliable diagnosis, effective treatment and prevention of diseases.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
3. Lymnaea cubensis, an experimental intermediate host for Fascioloides magna
- Creator:
- Vignoles, Philippe, Novobilský, Adam, Höglund, Johan, Kašný, Martin, Pankrác, Jan, Dreyfuss, Gilles, Pointier, Jean-Pierre, and Rondelaud, Daniel
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- giant liver fluke, experimental infections, Galba truncatula, snail generation, and snail susceptibility
- Language:
- English
- Description:
- Single-miracidium infections of Lymnaea cubensis (Pfeiffer) from Guadeloupe with the giant liver fluke Fascioloides magna (Bassi, 1875) (Digenea) were carried out during five successive snail generations to determine if this lymnaeid might sustain complete larval development of the parasite. Controls were constituted by a French population of Galba truncatula (Müller) (a single generation) infected according to the same protocol. It was recorded that prevalence and intensity of F. magna infection in L. cubensis progressively increased from F1 to F5 generations. Cercarial shedding of F. magna was noted only within F5 generation of L. cubensis. However, most measured parameters of infection in this species were significantly lower than those noted for G. truncatula and most L. cubensis died after a single shedding wave. Despite this, L. cubensis can be added to the list of potential intermediate hosts of F. magna.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
4. Peptidases of Trichobilharzia regenti (Schistosomatidae) and its molluscan host Radix peregra s. lat. (Lymnaeidae): construction and screening of cDNA library from intramolluscan stages of the parasite
- Creator:
- Dolečková, Kateřina, Kašný, Martin, Mikeš, Libor, Mutapi, Francisca, Stack, Colin, Mountford, Adrian P., and Horák, Petr
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Language:
- English
- Description:
- Trichobilharzia regenti is a neurotropic bird schistosome, causing cercarial dermatitis in humans. In this study, ZAP cDNA expression library from Radix peregra s. lat. hepatopancreases containing intramolluscan stages of T. regenti was constructed and screened using PCR with specific and degenerate primers, designed according to previously described serine and cysteine peptidases of other parasite species. Full-length sequences of cathepsins B1 and L, and two serine peptidases, named RpSP1 and RpSP2, were obtained. The protein-protein BLAST analysis and parallel control reactions with template from hepatopancreases of T. regenti non-infected snails revealed that only cathepsin B1 was of parasite origin. The remaining sequences were derived from the snail intermediate host, which implies that the initial source of parasite mRNA was contaminated by snail tissue. Regardless of this contamination, the cDNA library remains an excellent molecular tool for detection and identification of bioactive molecules in T. regenti cercariae.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public