A study was conducted from 2007 to 2010 to determine the status of the endemic loach species of Iran. So far 27 loach species belonging to two families (Nemacheilidae and Cobitidae) and eight genera have been recorded from 19 water basins of Iran. Eleven loach species are endemic to Iran. Oxynoemacheilus farsicus is considered as junior synonym of Oxynoemacheilus persus. The species Ilamnemacheilus longipinnis is known from a single specimen collected in the Tigris basin in 1978 and has to be considered as endemic but extinct in Iran. While some species like Paracobitis smithi are vulnerable due to their restricted and isolated habitat but drought, habitat alteration, extensive water extraction for irrigation and water pollution seems to be the major threatening factors to the loach survival in Iran. The Iran Department of Environment with the cooperation of Iranian Fisheries Research Organization must implement conservation programs to safeguard survival of Iran endemic loaches.