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2. Genetic diversity of Gobio gobio populations in the Cyech Republic and Slovakia, based on RAPD markers
- Creator:
- Mendel, Jan, Lusková, Věra, Halačka, Karel, Lusk, Stanislav, and Vetešník, Lukáš
- Type:
- article and TEXT
- Subject:
- RAPD, common gudgeon, diagnostic markers, and intraspecific diversity
- Language:
- English
- Description:
- The random amplified polymorphic DNA (RAPD) method was applied to eight Gobio gobio populations living in the rivers of the Czech Republic and Slovakia. The application of seven RAPD primers yielded eight fingerprint characteristics for the populations examined. Forty diagnostic markers have been identified, which can reliably identify the populations under study. Intrapopulation diversity varied between 0.26 and 0.38. A phenogram documented the close agreement of the particular populations with the geographic pertinence of their localities to the different sea basins.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
3. Genetic diversity of Misgurnus fossilis populations from the Czech Republic and Slovakia
- Creator:
- Mendel, Jan, Lusk, Stanislav, Koščo, Ján, Vetešník, Lukáš, Halačka, Karel, and Papoušek, Ivo
- Type:
- article and TEXT
- Subject:
- weather loach, endangered species, control region, cross-species amplification, and microsatellites
- Language:
- English
- Description:
- Monitoring of the analysed populations of the endangered fish species Misgurnus fossilis was conducted using microsatellite analysis and sequencing of a part of the control region. Absolutely first microsatellite markers for weather loach were found. Six polymorphic microsatellite loci were prepared, five of which were tested. Number of alleles per a locus ranged from 3 to 5. All studied populations are differentiated one from another (FST= 0.205 - 0.367). All sampled populations contained unique alleles. Sequential analysis of the mitochondrial control region showed great haplotype similarity of the studied populations which come from one widely spread haplotype H_1, and thus suggested possible hypothesis of recent spreading from one source.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
4. Genetic diversity of Sabanejewia balcanica (Actinopterygii: Cobitidae) in the western Balkans and comparison with other regions
- Creator:
- Marešová, Eva, Delić, Antun, Kostov, Vasil, Marić, Saša, Mendel, Jan, and Šanda, Radek
- Type:
- article and TEXT
- Subject:
- Balkan golden loach, cytochrome b, mitochondrial DNA, and Balkans region
- Language:
- English
- Description:
- Inter-population variability within Sabanejewia populations from the western Balkans, and their phylogenetic position in comparison to other European populations were investigated. Of 79 samples analysed, 51 unique haplotypes were identified. Network analysis divided thirteen populations from five river basins into two clusters: cluster I was composed of populations from the Vardar drainage and tributaries of the neighbouring River Morava (River Danube basin), while cluster II contained the River Timok (eastern Serbia) and all the River Sava populations. The only locality that housed haplotypes of both clusters was the River Kutinska reka in the upper Morava basin. When compared with the haplotypes reported in former studies, both clusters fell within the ‘Danubian-Balkanian complex’. Cluster II was included in the most heterogeneous sub-lineage S. montana – S. bulgarica – S. balcanica (III), while cluster I was related to the sub-lineages S. doiranica – S. balcanica (II) and S. balcanica (VI). Recently published haplotypes from the Croatian Sava (rivers Petrinjčica and Rijeka) and Drava (rivers Drava and Voćinska) basins, as well as Czech and Slovak individuals from the Danube and Tisza river basins were included. The River Drava showed the same population subdivision as the River Kutinska reka.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/