Stages of the life cycle of Ascocotyle (Phagicola) angeloi Travassos, 1928 were experimentally obtained, from cercariae from naturally infected Littoridina castellanosae (Gaillard) collected in artificial ponds in the Zoological Garden of Buenos Aires. Metacercariac were found encysted mainly in muscles and ovary, but also in other parts of the body of naturally and experimentally infected fish Jenynsia lineata (Jenyns) (Atheriniformes: Jenynsidae). Adults were obtained experimentally from chicks and mice. Ascocotyle (Phagicola) angeloi, rcdescribed in the present paper, is distinguished from other species of the subgenus by the two rows of oral spines, each with 14 spines. The characteristics of the studied cercaria corresponds to those of the subgenus Phagicola.