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2. Mohl český král Ladislav Pohrobek trpět hypofosfatemickou osteomalácií vyvolanou nádorovým onemocněním? /
- Creator:
- Povýšil, C.
- Subject:
- Ladislav,, nemoci, české země 1437-1471, and choroby, epidemie
- Language:
- Czech
- Description:
- Could the Czek King Ladislaus, Posthumous, Have Suffered from Hypophosphacaenic Osteomalacia Caused by Neoplastic Discase?
- Rights:
- unknown
3. New developments in understanding the histological structure of human ear cartilage
- Creator:
- Kaňa, M., Kaňa, R., and Povýšil, C.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- auricular cartilage, layered arrangement of chondrocytes, immunophenotype of chondrocytes, and maintenance of fixed shape of the pinna
- Language:
- English
- Description:
- Histological, immunohistochemical and molecular examination of bioptic samples of 30 normal adult auricular cartilages and small samples from 6 ear cartilages from aborted foetuses was performed. The adult cartilage was the tissue with minimal proliferative activity, which we were able to confirm with antibodies against Ki67 in contrast to a high proliferative activity in the auricular cartilage of foetal tissues. It may therefore be presumed that the process of foetal tissue maturation is undoubtedly associated with a significant reduction in proliferative activity. The mature lamella of the adult auricular cartilage has a histological tri-lamellar structure. There are a great number of elastic fibres in the intercellular matrix of the central zone, which are conversely present in only small amounts in both peripheral layers. While the external layer of the concave surface of the cartilage contains a fewer number of oval elements, the external layer of the convex side is composed of numerous fusiform chondrocytes. and Antibodies against various subtypes of S-100 protein showed that auricular chondrocyte activity is modified depending on the configuration of individual distinct zones (isoforms A1, A6, B2 and P were positive in all layers, isoforms A2 and A2 in peripheral zones). The most active cells metabolically are most likely chondrocytes in both external layers adjacent to the perichondrium. We have also demonstrated α-smooth muscle actin (SMA)-positive chondrocytes in both peripheral layers of the auricular cartilage adjacent to the perichondrium. In addition, we found definite differences in the distribution of actin-positive cells depending on the external shape of the pinna. The majority of these fusiform cells were localised primarily in the areas of great curvature of the pinna, especially the convex side, as mentioned above. On the basis of these unique structural features we assume that the ear cartilage may embody an example of the socalled intelligent biological material, which has its internal structure made in such a way as to more easily develop and yet still maintain all the shape characteristics of the human auricle. The knowledge of these specific structural characteristics is important especially for use of auricular cartilage in auricular reconstruction.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
4. The localisation of TPPS4 in some organs and its possible nephrotoxicity in rats
- Creator:
- Zima, T., Jirka, M., Jirsa jr., M., Bradová, V., Stejskal, J., Žabka, J., Povýšil, C., and Janebová, M.
- Type:
- article, model:article, and TEXT
- Subject:
- nephrotoxicity, NAG, organ localisation, meso-tetra-(4-sulfonatophenyl)-porphine (TPPS4), renal function, and photodynamic therapy
- Language:
- English
- Description:
- Photodynamic therapy (PDT) is now being used more frequently in carefully selected cases of malignancies. The drugs used for PDT are mostly derivatives of haematoporphyrine (HPD) and its active component photofrine II. Another compound prepared by total synthesis is meso-tetra-(4-sulfonatophenyl)-porphine (TPPS4) but its application in human medicine was rejected because of its neurotoxicity. Our TPPS4 was prepared by the method of Busby et al. in the modification of Jirsa and Kakaë (1987). This product is purer and without neurotoxic effects. In this study, we concentrated our attention on the effect of TPPS4 on nephrotoxicity and its accumulation in some organs. As the parameters of toxic kidney damage we used urine levels of N-acetyl-beta-D-glucosaminidase (NAG), serum creatinine levels, glomerular filtration rate (GFR) and proteinuria. TPPS4 was administered i.v. in a dose of 25 mg/kg b.w. The animals were observed for 21 days after drug application. Urine and blood samples were collected over 24-hour periods on days 0, 5 and 21. The serum creatinine level was significantly higher only on day 5 (65.0±1.46 /zmol/1 vs 56.5±2.69 ^mol/1 on day 0, p<0.05). There were no significant changes in GFR, proteinuria or NAG activity in the urine during the experiment. AST serum activity was increased. We determined the concentration of TPPS4 (pmol/mg w.w.) in rat organs on the 21st day after the injection. The concentration of TPPS4 was high in kidneys (30.8 ±5.5), liver (13.5 ±2.0), lungs (11.7 ±4.6) and spleen (9.7 ±1.5), while the concentration in heart and brain was low. We conclude that TPPS4 has the highest concentration in the kidney 21 days after its administration and does not exert any nephrotoxic effects during this period.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public