Hydrological processes play important roles in soil erosion processes of the hillslopes. This study was conducted to investigate the hydrological processes and the associated erosional responses on the purple soil slope. Based on a comprehensive survey of the Wangjiaqiao watershed in the Three Gorges Reservoir, four typical slope gradients (5°, 10°, 15°and 20°) were applied to five rainfall intensities (0.6, 1.1, 1.61, 2.12 and 2.54 mm·min-1). The results showed that both surface and subsurface runoff varied greatly depending on the rainfall intensity and slope gradient. Surface runoff volume was 48.1 to 280.1 times of that for subsurface runoff. The critical slope gradient was about 10°. The sediment yield rate increased with increases in both rainfall intensity and slope gradient, while the effect of rainfall intensity on the sediment yield rate was greater than slope gradient. There was a good linear relationship between sediment yield rate and Reynolds numbers, flow velocity and stream power, while Froude numbers, Darcy-Weisbach and Manning friction coefficients were not good hydraulic indicators of the sediment yield rate of purple soil erosion. Among the three good indicators (Re, v and w), stream power was the best predictor of sediment yield rate (R2 = 0.884). Finally, based on the power regression relationship between sediment yield rate, runoff rate, slope gradient and rainfall intensity, an erosion model was proposed to predict the purple soil erosion (R2 = 0.897). The results can help us to understand the relationship between flow hydraulics and sediment generation of slope erosion and offer useful data for the building of erosion model in purple soil.
The cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel contains 12 transmembrane (TM) regions that are presumed to form the channel pore. However, t here is no direct evidence clearly illustrating the involvement of these transmembr ane regions in the actual CFTR pore structure. To obtain insight into the architecture of the CFTR channel pore, we used patch clamp recording techniques and a strategy of comutagenesis of two potential pore-forming transmembrane regions (TM1 and TM6) to investigate the collaboration of these two TM regions. We performed a range of specific functional assays comparing the single channel conductance, anion binding, and anion selectivity properties of the co -mutated CFTR variants, and the results indicated that TM1 and TM6 play vital roles in forming the channel pore and, thus, determine the functional properties of the channel. Furthermore, we provide d functional evidence that the amino acid threonine (T338) in TM6 has synergic effects with lysine (K95) in TM1. Therefore, we propose that these two residues have functional collaboration in the CFTR channel pore and may collectively form a selective filter ., F. Qian, L. Liu, Z. Liu, C. Lu., and Obsahuje bibliografii