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Start Over Creator Ronzhina, M.

1. Application of the optical method in experimental cardiology: action potential and intracellular calcium concentration measurement

Creator:
Ronzhina, M., Čmiel, V., Janoušek, O., Jana Kolářová, Marcela Nováková, Babula, P., and Ivo Provazník
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, optical method, fluorescent dyes, action potential, calcium concentration, 14, and 612
Language:
English
Description:
It has been shown that, in addition to conventional contact electrode techniques, optical methods using fluorescent dyes can be successfully used for cardiac signal measurement. In this review, the physical and technical fundamentals of the method are described, as well as the properties of the most common systems for measuring action potentials and intracellular calcium concentration. Special attention is paid to summarizing limitations and trends in developing this method., M. Ronzhina, ... [et al.]., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Motion artefact in voltage-sensitive fluorescent dye emission during repeated ischemia of isolated heart

Creator:
Janoušek, O., Jana Kolářová, Ronzhina, M., Marie Nováková, and Ivo Provazník
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, motion artefact, ischemia, isolated rabbit heart, voltage-sensitive dye, 14, and 612
Language:
English
Description:
Motion artefact (MA) in voltage-sensitive fluorescent signals causes significant debasement of action potential. During ischemia and reperfusion in isolated rabbit heart, this artefact develops in a manner which may be described by the time of its onset, level, and shape. The MA during ischemia: (a) may become substantial with approximately two minutes delay after establishing global ischemia; (b) may be almost twice as high as the physiological action potential and decreases both with time and repetition of ischemia; (c) the MA shape is unpredictable and depends on individual rabbit., O. Janoušek, ... [et al.]., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Tolerance of isolated rabbit hearts to short ischemic periods is affected by increased LV mass fraction

Creator:
Marie Hlaváčová, Veronika Olejníčková, Ronzhina, M., Tibor Stračina, Oto Janoušek, Nováková, M., Petr Babula, Kolářová, J., Ivo Provazník, and Hana Paulová
Format:
print, bez média, and svazek
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, srdce, ischemie, heart, ischemia, 4-hydroxy-2-nonenal, electrogram, rabbit isolated heart, LV mass, global ischemia, 14, and 612
Language:
English
Description:
Hypertrophied hearts are known for increased risk of arrhythmias and are linked with reduced ischemic tolerance. However, still little is known about state characterized only by increased left ventricle (LV) mass fraction. Seventeen isolated rabbit hearts with various LV mass were divided into two groups according to LV weight/heart weight ratio (LVW/HW ratio), namely group H and L (with higher and lower LVW/HW ratio, respectively) and underwent three short cycles of global ischemia and reperfusion. The differences in electrogram (heart rate, QRSmax, mean number, onset and dominant form of ventricular premature beats) and in biochemical markers of myocardial injury (creatine kinase, lactate dehydrogenase - LDH) and lipid peroxidation (4-hydroxy-2-nonenal - 4-HNE) were studied. As compared to group L, hearts in group H exhibited lower tolerance to ischemia expressed as higher incidence and severity of arrhythmias in the first ischemic period as well as increase of LDH and 4-HNE after the first reperfusion. In the third cycle of ischemia-reperfusion, the preconditioning effect was observed in both electrophysiological parameters and LDH release in group H. Our results showed consistent trends when comparing changes in electrograms and biochemical markers. Moreover, 4-HNE seems to be good potential parameter of moderate membrane alteration following ischemia-reperfusion injury., M. Hlaváčová, V. Olejníčková, M. Ronzhina, T. Stračina, O. Janoušek, M. Nováková, P. Babula, J. Kolářová, I. Provazník, H. Paulová., and Obsahuje bibliografii
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public