In the current study 744 cloacal samples were collected from mallards (Anas platyrhynchos) in the Czech Republic and tested for the presence of influenza virus between 2008 and 2010. Of the total number of 744 mallards tested nine were positive (prevalence 1.2 %) for influenza virus. All the mallards were up to 1.5 years old and the majority (89 %) were killed by hunters.