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1172. Mass spectrometry analyses of rat 2b myosin heavy chain isoform

Creator:
Jitka Žurmanová, Daniela Horníková, František Půta, Petr Novák, Jan Říčný, and Tomáš Soukup
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Obecná zoologie, fyziologie živočichů, mikrobiologie, spektrometrie, animal physiology, microbiology, spectrometry, rat muscle myosin, myosin heavy chain 2b isoform, MALDI TOF mass spectrometry, ESI LC/MS/MS mass spectrometry, 2, and 591
Language:
English
Description:
We have separated 2b myosin heavy chain (MyHC) isoform from the rat extensor digitorum longus muscle by SDS-PAGE and analyzed it by two subsequent mass spectrometry techniques. After tryptic digestion, the obtained peptides were identified by Matrix-Assisted Laser Desorption/Ionisation reflectron Time of Flight mass spectrometry (MALDI-TOF MS) and sequenced by Liquid chromatography tandem mass spectrometry (ESI LC/MS/MS). The analyzed peptides proportionally covered 30 % of the 2b MyHC isoform sequence. The results suggest that the primary structure is identical with the highest probability to a NCBI database record ref|NP_062198.1|, representing the last updated record of rat 2b isoform. Nonetheless, four peptides carrying amino acid substitution(s) in comparison with the NCBI database record were identified., J. Žurmanová, D. Maláčová, F. Půta, P. Novák, J. Říčný, T. Soukup., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

1173. Mating advantage of short-winged over long-winged adult males in the cricket Velarifictorus ornatus (Orthoptera: Gryllidae)

Creator:
Zhao, Lv-Quan, Chai, Huai-Lin, Wu, Hong-Jun, and Zhu, Dao-Hong
Format:
print, počítač, and online zdroj
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Zoologie, rovnokřídlí, cvrčkovití, Orthoptera, Gryllidae, Velarifictorus ornatus, mating competition, sing song, trade-off, wing polymorphism, 2, and 59
Language:
English
Description:
The trade-off between flight capability and reproduction is well known in adult males of insects with wing dimorphism but the reproductive advantage of short-winged (SW) males over long-winged (LW) males appears to vary across insect taxa. In the present study, we determined the difference in the mating ability of SW and LW males of Velarifictorus ornatus (Orthoptera: Gryllidae) in order to evaluate whether the SW male morph has a reproductive advantage. We found that the choice of a mate depended on the female. Compared with LW males, SW males had an obvious mating advantage when both SW and LW males courted females simultaneously, and that dealation significantly enhanced the mating ability of LW males. Losing the ability to produce songs reduced the mating advantage of SW males, thereby indicating that the greater mating advantage of SW males was related to the attractiveness of the song. In addition, the difference in the mating ability of LW and SW males was not related to body size or age. These results indicate that SW males of V. ornatus have a mating advantage over their LW counterparts because their underdeveloped flight muscles allow them to devote more resources to reproduction., Lv-Quan Zhao, Huai-Lin Chai, Hong-Jun Wu, Dao-Hong ZhuU., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

1174. Mating alters the rate of development of ovarioles in the ladybird, Propylea dissecta (Coleoptera: Coccinellidae)

Creator:
Shahid, Mhd, Siddiqui, Arshi, Omkar, and Mishra, Geetanjali
Format:
print, počítač, and online zdroj
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Zoologie, brouci, slunéčkovití, věk, beetles, Coccinellidae, age, Coleoptera, Propylea dissecta, mating status, ovariole development, egg maturation, egg load, 2, and 59
Language:
English
Description:
The influence of female mating status on ovarian development of the ladybird, Propylea dissecta (Mulsant) (Coleoptera: Coccinellidae), was investigated under laboratory conditions. We assessed the extent to which ovariole development was affected by mating and for that we initially created a base line by observing age specific ovariole development. Results show that the number of follicles in each ovariole increased with the age of both virgin and mated females up to the age of 3 days, thereafter, no increase in number of follicles was recorded. Ovariole width also increased with age in both virgin and mated females up to 4 days, thereafter, no increase in ovariole width was recorded. The ovariole width of mated females was significantly greater than that of virgin females. Egg maturation and the egg load started to increase at the age of 8 days in virgin females. Thereafter, it increased with increase in female age. While in mated females, immature eggs were recorded in their ovarioles from the age of 1 to 2 days. In mated females, however, the increase in the number of mature eggs per ovariole and egg load started when they were 3 days old. Egg load continuously increased with increasing female age., Mhd Shahid, Arshi Siddiqui, Omkar, Geetanjali Mishra., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

1175. Measurements of reflectance and fluorescence spectra for nondestructive characterizing ripeness of grapevine berries

Creator:
Navrátil, M. and Buschmann, C.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
fotosyntéza, photosynthesis, Cabernet Sauvignon, CIE 1931, plant pigments, Riesling, 2, and 581
Language:
Multiple languages
Description:
In vivo reflectance and fluorescence spectra from berry skins of a white (Riesling) and red (Cabernet Sauvignon) grapevine variety were measured during a ripening season with a new CMOS radiometer instrument. Classical reference measurements were also carried out for a sugar content of the berry juice [°Brix] and pigment contents (chlorophyll a and b, carotenoids, anthocyanins) from methanol extracts of the berry skin. We showed that the colours and the spectra analysed from them could be taken as an unambiguous indicator of grapevine ripening. Reflectance spectra, which were affected by the content of pigments (chlorophylls and anthocyanins), effects of surface (wax layers), and tissue structure (cell size) of the berries well correlated (R2 = 0.89) with the °Brix measurements of the berries. The fast data acquisition of both reflectance and fluorescence spectra in one sample with our radiometer instrument made it superior over the time-consuming, traditional, and mostly destructive chemical analysis used in vine-growing management., M. Navrátil, C. Buschmann., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

1176. Mechanismy vývinu hypertenze závislé na soli

Creator:
Michal Pravenec
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Biologické vědy, hypertenze, kuchyňská sůl, hemodynamika, fyziologie, hypertension, table salt, hemodynamics, physiology, vliv soli, mineralokortikoidy, aldosteron, mineralocorticoids, 2, and 57/59
Language:
Czech
Description:
Hypertenze závislá na soli patří mezi nejčastější rizikové faktory kardiovaskulárních onemocnění. U většiny případů je příčina tohoto onemocnění neznámá, avšak významný podíl hypertenzních jedinců citlivých k soli má zvýšené hladiny mineralokortikoidů. V tomto přehledném článku popisujeme hemodynamické abnormality a mechanismy odpovědné za vývin této formy hypertenze., Salt-dependent hypertension is a leading cause of cardiovascular diseases. In most cases, the etiology is unknown, but it has been estimated that a significant percentage of salt-sensitive hypertensive individuals have mineralocorticoid excess. In this review, we describe hemodynamic abnormalities and mechanisms responsible for initiation of this form of hypertension., and Michal Pravenec.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

1177. Mechorosty České republiky

Creator:
Jan Kučera
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Botanika, flóra, mechy, mechorosty, Česko, 2, and 58
Language:
Czech
Description:
Bryophytes, with their more than 860 species, represent more than a quarter of higher plants native to the CR. They can reveal much more about the ecosystems they live in than we would expect, as their physiological contact with the surroundings is much closer than that of the vascular plants. and Jan Kučera.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

1180. Medúzka sladkovodní: rosolovitý návštěvník z Číny

Creator:
Adam Petrusek
Format:
print, bez média, and svazek
Type:
article, text, články, journal articles, model:article, and TEXT
Subject:
Biologické vědy, žahavci, polypovci, sladkovodní živočichové, Cnidaria, Hydrozoa, freshwater animals, medúzka sladkovodní, Craspedacusta sowerbii, Freshwater Jellyfish, 2, and 57/59
Language:
Czech
Description:
Medúzka sladkovodní (Craspedacusta sowerbii) je jediným zástupcem rosolovitého zooplanktonu v našich vodách. Článek popisuje historii zkoumání, životní cyklus a ekologii tohoto u nás nepůvodního druhu, který byl do celého světa zavlečen z Číny., The Freshwater Jellyfish (Craspedacusta sowerbii) is the only representative of gelatinous zooplankton in Czech waters. This article describes the body of research on the invasive species, which has spread from China all over the world. The article also includes a description of the species´ life cycle and ecology., and Adam Petrusek.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

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